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Binding of Calcium Oxalate and Apatite Crystals to Renal Papillary Collecting Tubule Cells in Primary Culture

  • R. Riese
  • J. Wiessner
  • J. Kleinman
  • G. Mandel
  • N. Mandel

Abstract

The attachment of microcrystallites within the renal papilla may be an important component of the pathophysiology of urolithiasis (1–3). The current study utilizes rat inner-papillary collecting tubule (RPCT) cells in primary culture to investigate how calcium oxalate monohydrate (CaOx) and apatite (AP) crystals interact with cell surfaces. We previously reported (4, 5) an increased CaOx crystal adherence to aggregated cells as compared to monolayer cells in the RPCT cell cultures. Also, we demonstrated the specificity of CaOx adherence to these cultures and developed a mathematical model to analyze the concentration-dependent binding of microcrystallites to the RPCT cells (5). This study is a continuation of our previous study (5) examining the concentration-dependent binding of CaOx and AP microcrystallites to the RPCT cells, and compares the binding of these two crystalline moieties in greater detail.

Keywords

Calcium Oxalate CaOx Crystal Calcium Oxalate Monohydrate Renal Papilla Kidney Stone Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B Finlayson and F Reid, The expectation of free and fixed particles in urinary stone disease, Invest. Urol. 15: 442 (1978).PubMedGoogle Scholar
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    L Cifuentes-Delatte, JLR Minon-Cifuentes, and JA Medina, Papillary stones: calcified renal tubules in Randall’s plaques, J. Urol. 133: 490 (1985).PubMedGoogle Scholar
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    R Randall, Papillary pathology as precursor of primary renal calculus, J. Urol. 44: 580 (1940).Google Scholar
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    JH Wiessner, JG Kleinman, SS Blumenthall, JC Garancis, GS Mandel, and NS Mandel, Calcium oxalate crystal interaction with rat papillary collecting tubule cells, J. Urol. 138: 640 (1987).PubMedGoogle Scholar
  5. 5.
    RJ Riese, JW Riese, JG Kleinman, JH Wiessner, GS Mandel, and NS Mandel, Specificity in calcium oxalate adherence to papillary epithelial cells in culture, Am. J. Physiol. In press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • R. Riese
    • 1
  • J. Wiessner
    • 1
  • J. Kleinman
    • 1
  • G. Mandel
    • 1
  • N. Mandel
    • 1
  1. 1.The VA Medical CenterThe Medical College of WisconsinMilwaukeeUSA

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