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Urolithiasis pp 549–551Cite as

Microdetermination of Urinary Constituents by Vertical Light-Path Photometry in Microplates

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Abstract

Automated photometric measurements in microtiter plates have been used for some years in our laboratory in order to determine kinetic parameters of crystal growth in gels (1). We have now applied vertical light-path photometry in 96-well microplates to quantify various soluble constituents of urine and serum which are relevant to diagnosis and basic research of urinary stone formation.

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References

  1. W Achilles, Crystallization in gel matrices: a new experimental model of calcium stone formation, Contr. Nephrol. 58: 59 (1987).

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  2. PF Wright, DEJ Gall, and WA Kelly, Effect of meniscus formation and duplicate sample placement configurations on the variability of measurement by three microtiter plate photometers, J. Immun. Meth. 81: 83 (1985).

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  3. JDE Young, CC Liu, LG Leong, A Damiano, PM Persechini, and ZA Cohn, An automated colorimetric microassay system for rapid and quantitative determination of soluble enzymatic activities, Biotechniques 5: 572 (1987).

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© 1989 Springer Science+Business Media New York

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Achilles, W., Schalk, C., Berwernick, J., Rodeck, G. (1989). Microdetermination of Urinary Constituents by Vertical Light-Path Photometry in Microplates. In: Walker, V.R., Sutton, R.A.L., Cameron, E.C.B., Pak, C.Y.C., Robertson, W.G. (eds) Urolithiasis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0873-5_169

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  • DOI: https://doi.org/10.1007/978-1-4899-0873-5_169

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0875-9

  • Online ISBN: 978-1-4899-0873-5

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