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Urolithiasis pp 543-545 | Cite as

A New Method for Glycolate Determination in Plasma and Urine

  • J. Costello
  • J. Brothis
  • J. Humpierres
  • D. M. Landwehr

Abstract

A new enzymatic-colorimetric method for determination of plasma and urinary glycolate is described. Plasma is ultrafiltered and urine is charcoal-treated prior to analysis. Glycolate loss is determined following incubation with glycolate oxidase.

Keywords

Glycolic Acid Glyoxylic Acid Primary Hyperoxaluria Analytical Recovery Glycolate Oxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    S Dagley and A Rogers, Estimation of glycolic acid, Biochim. Biophys. Acta 12: 591 (1953).Google Scholar
  2. 2.
    GP Kasidas and GA Rose, A new enzymatic method for the determination of glycolate in urine and plasma, Clin. Chim. Acta 96: 25 (1979).PubMedCrossRefGoogle Scholar
  3. 3.
    R Bais, JM Nairn, N Potezny, AM Rofe, RAJ Conyers, and A Bar, Urinary glycolate measured by use of (S)-2-hydroxy-acid oxidase, Clin. Chem. 31: 710 (1985).PubMedGoogle Scholar
  4. 4.
    KE Richardson and NE Tolbert, Oxidation of glyoxylic acid to oxalic acid by glycolic acid oxidase, J. Biol. Chem. 236: 1280 (1961).PubMedGoogle Scholar
  5. 5.
    N Niederwieser, A Matasovic, and EP Leumann, Glycolic acid in urine. A colorimetric method with values in normal adult controls and in patients with primary hyperoxaluria, Clin. Chim. Acta 89: 13 (1978).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • J. Costello
    • 1
  • J. Brothis
    • 1
  • J. Humpierres
    • 1
  • D. M. Landwehr
    • 1
  1. 1.Allegheny General Hospital and Allegheny-Singer Research InstitutePittsburghUSA

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