Oxalate Transport Studies in Intestinal and Renal Brush-Border-Membrane Vesicles in Pyridoxine-Deficient Rats
Nutritional pyridoxine deficiency in rats leads to hyperoxaluria, a major risk factor for calcium-oxalate lithiasis. Oxalate transport across intestinal and renal brush-border-membrane vesicles (BBMV) was studied to understand the mechanism of hyperoxaluria. Acute, subclinical, and chronic pyridoxine deficiencies were induced in male weanling rats. Intestinal and renal BBMV were prepared and tested for purity, vesicularity, and integrity. Oxalate uptake was studied by a rapid filtration technique (1).
KeywordsMajor Risk Factor Brush Border Membrane Postgraduate Institute Uptake Intestine Pyridoxine Deficiency
- 1.U Hopfer, K Nelson, J Perotto, and KJ Isselbacher, Glucose transport in isolated brush border membrane from rat small intestine, J. Biol. Chenu 248:25 (1973).Google Scholar