Abstract
Recent evidence from several laboratories has demonstrated that human breast cancer cell lines produce a variety of polypeptide growth factors, including platelet derived growth factor (PDGF), insulin like growth factor (IGFI), transforming growth factors alpha and beta (TGFα and TGFβ), mammary-derived growth factor I (MDGFI), mammary-derived growth factor II (MDGFII), gastrin releasing peptide (GRP), human mammary tumor growth factor (h.MTGF) and epidermal growth factor (EGF). It has been assumed that production of the factors was a pathological phenomenon, one accompanying neoplastic transformation. However, we and others have shown that most of these are normal components of milk. Consequently, we postulated that the tumor cell-derived factors might be normal glandular secretory products.
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W.R. Kidwell, M. Bano and D.S. Salomon, Growth control of normal mammary cells on collagen in serum-free medium. In: “Methods for serum-Free Culture of Cells of the Endocrine System,” D. Barnes, D. Sirbasku and G.H. Sato, eds., Alan R. Liss, Inc., New York, 1984, pp 105–125.
I. Perroteau, W.R. Kidwell, R. Pardue, M. Debertoli and D.S. Salomon, Immunoreactive alpha-transforming growth factor in human breast cancer extracts and cell lines, Breast Cancer Res. Treat. 7:201 (1986).
D.S. Salomon, J.A. Zwiebel, M. Bano, I. Losonczy, P. Fehnel and W.R. Kidwell, Presence of transforming growth factors in human breast cancer cells, Cancer Res. 44:4069 (1984).
K.K. Huff, D. Kaufman, K.H. Gabbay, E.M. Spencer, M. Lippman and R.B. Dickson, Secretion of an insulin-like growth factor-l-related protein by human breast cancer cells, Cancer Res. 46:4613 (1986).
D.S. Salomon, I. Perroteau, W.R. Kidwell, J. Tarn and R. Derynck, Loss of growth responsiveness to EGF and enhanced production of alphatransforming growth factor in ras-transformed mouse mammary cells, J. Cell Phys. In press.
R. Derynck, D.V. Goeddel, A. Ullrich, J. Gutterman, R. Williams, T.S. Bringman and W.H. Berger, Synthesis of mRNAs for transforming growth factors and EGF receptor by human tumors. Proc. Natl. Acad. Sci. USA. In press.
W.R. Kidwell S. Liu and O.S. Salomon, Ovariectomy reduces the level of transforming growth factor alpha and its mRNA in primary, 7, 12-DMBA-induced rat mammary tumors, Breast Cancer Res. Treat. In press.
R.B. Dickson, K.K. Huff, E.M. Spencer and M.E. Lippman, Induction of epidermal growth factor-related peptides by 17 beta-estradiol in MCF-7 cells, Endocrin. 118:138 (1986).
D.S. Salomon, W.R. Kidwell, G.S. Smith and G.I. Bell, Presence of alpha transforming growth factors in human breast tumors and milk, Cancer Res. 26:198 (1985).
J.A. Zwiebel, M. Bano, D.S. Salomon and W.R. Kidwell, Partial purification of transforming growth factors from human milk, Cancer Res. 46:933 (1986).
J.P. Tarn, Physiological effects of TGF in the new-born mouse. Sci. 229:673 (1985).
W.R. Kidwell and D.S. Salomon, Growth factors in human milk, in: “Minor Protein Constituents in Human Milk,” S. Atkinson and B. Lonnerdahl, eds., CRC Reviews, Boca Raton. In press.
J. Scott, M. Urdea, M. Quiroga, R. Sanchez-Pescador, N. Fong, M. Selby, W. Rutter, and G.I. Bell, Structure of a mouse submaxillary gland mRNA coding for EGF and seven related peptides. Sci. 221:236 (1983). 1983.
R.A. Gol-Winckler, A. Simenon, H. Dijkmas and J.A. Martial, Presence of mRNA encoding polypeptide growth factors and EGF receptor in primary human breast cancer, Second Annual Meeting on Oncogenes, Hood College, Frederick, Maryland, p. 93, July 1986.
J. Collette, J-C. Hendrick, and P. Franchimont, Presence of lactalbumin, epidermal growth factor, epithelial membrane antigen and gross cystic disease fluid protein in breast cyst fluid, Cancer Res. 46:3728 (1986).
J.M. Beadmore, D.I. Lewis-Jones and R.C. Richards, Urogastrone and lactose concentrations in precolostrum, colostrum and milk, Peadr. Res. 17:825 (1983).
K. Mori, M. Kurobe, S. Furukawa, K. Kubo and K. Hayaishi, Human breast cancer cells synthesize and secrete EGF-like immunoreactive factor in culture, Biochem. Biophys. Res. Commun. 136:300 (1986).
R.B. Rall, J. Scott and G.I. Bell, Mouse pre-proEGF synthesis by kidney and other tissues, Nature 313:228 (1985).
S. Okamoto and T. Oka, Evidence for a physiological function of epidermal growth factor: pregestational sialadectomy of mice decreases milk production and increases offspring mortality during the lactation period, Proc. Natl. Acad. Sci. USA 81: 6059 (1985).
E.W. Gresik, H. Van Der Noen and T. Barka, Transport of 125I-EGF into milk and effect of sialadectomy on milk EGF in mice, Amer. J. Physiol. 247:E349 (1984).
M. Bano, D.S. Salomon and W.R. Kidwell, Purification of a mammary derived growth factor from human milk and human mammary tumors, J. Biol. Chem. 260:5745 (1985).
M.S. Wicha, L.A. Liotta, S. Garbisa and W.R. Kidwell, Basement membrane collagen requirements for growth and attachment of mammary epithelium, Exp. Cell Res. 124:181 (1979).
M.S. Wicha L.A. Liotta, B.K. Vonderhaar and W.R. Kidwell, Effects of inhibition of basement membrane collagen deposition on rat mammary gland development, Dev. Biol. 80:253 (1980).
W.L. Lewko, L.A. Liotta, M.S. Wicha, B.K. Vonderhaar and W.R. Kidwell, Sensitivity of N-nitrosomethylurea-induced rat mammary tumors to cis-hydroxyproline, an inhibitor of collagen production. Cancer Res. 41:2855 (1981).
M. Bano, D.S. Salomon and W.R. Kidwell, Detection and partial characterization of collagen synthesis stimulating activities in mammary adenocarcinomas, J. Biol. Chem. 258:2729 (1983).
R. Pircher, D. Lawrence and P. Julien, Latent β-transforming growth factor in non-transformed and Kirsten sarcoma virus-transformed normal rat kidney cells, clone 49F, Cancer Res. 44:5538 (1984).
Y. Shing and M. Klagsbrun. Human and bovine milk contain two different sets of growth factors, Endocrin. 115:273 (1984).
G.D. Janke and L.H. Lazarus, Bombesin-like immunoreactive peptide in bovine milk, Proc. Natl. Acad. Sci. 81:578 (1984).
R. Ekman, S. Iversson and L. Jansson, Bombesin, neurotensin and proγ-melanotrophin immunoreactivity in human milk, Reg. Peptid. 10:99 (1985).
G. Guadino, M.E. DeBertoli and L.H. Lazarus, Bombesin-like immunoreactivity in human mammary tumors, 5th Internatl. George Washington Univ. Spring Symposium Proc, p 28, 1985.
G. Gaudino, M.E. DeBertoli and L.H. Lazarus, A bombesin-related peptide in experimental mammary tumors of rats, N.Y. Acad. Sci. In press.
K.D. Brown and D.M. Blakeley, Partial purification and characterization of a growth factor from goat colostrum, Biochem. J. 219:609 (1984).
E. Rosengurtz, J. Sinnett-Smith and J. Taylor-Papadimitriou, Production of PDGF-like growth factor by breast cancer cell lines, Int. J. Cancer 36: 247 (1985).
J.M. Rowe, S. Kasper, R. Shiu and H.G. Friesen, Purification and characterization of a human mammary tumor-derived growth factor, Cancer Res. 46:1408 (1986).
S. Cevreska, V.P. Kouacev, M. Stanovski and E. Kalamares, The presence of immunologicaily active insulin in milk of women during the first week of lactation and its relation to plasma insulin concentration. God. Zg. Med. Fak Skopje, 21:35 (1975).
S.K. Sinha and A.A. Yunis, Isolation of a colony stimulating factor from human milk, Biochem. Biophys. Res. Commun. 114:797 (1983).
R.C. Baxter, Z. Zaltsman and J. Turtle, Immunoreactive somatomedin C/IGFI and its binding protein in human milk, Endocrin. 58:955 (1984).
F.D. Bohmer, W. Lehmann, H. Eberhardt, P. Langen and R. Grosse, Purification of a growth inhibitor for ehrlich ascites mammary carcinoma cells from bovine mammary glands, Exp. Cell Res. 150:466 (1984).
P. Langen, W. Lehmann, H. Graetz, F.D. Bohmer and R. Grosse, Is ribonucleotide reductase in Ehrlich ascites mammary tumor cells the target of a growth inhibitor purified from bovine mammary gland?, Biochem. Biophys. Acta 43:1377 (1984).
B. Westley and H. Rochefort, A secreted glycoprotein induced by estrogen in human breast cancer cell lines, Cell 20:352 (1980).
M. Garcia, F. Capony, D. Derocq, D. Simon, B. Pau and H. Rochefort, Characterization of monoclonal antibodies to estrogen-regulated Mr 52,000 glycoprotein and their use in MCF-7 cells, Cancer Res. 45:709 (1985).
I. Touitou, M. Garcia, B. Westley, F. Capony and H. Rochefort, Effect of tunacamycin and endoglyciosidase H and F on the estrogen regulated 52,000 Mr protein seecreted by breast cancer cells, Biochemie 67:1257 (1985).
M. Morisset, F. Capony and H. Rochefort, The estrogen regulated 52,000 Mr protein is a cathepsin-1 ike protease, Biochem. Biophys. Res. Commun. In press.
M. Garcia, G. Salazar-Retana, G. Richer, J. Oomergue, F. Capony, H. Pujol, F. Laffargue, B. Pau and H. Rochefort, Immunohistichemical detection of estrogen regulated 52,000 mol wgt protein in primary breast cancer but not normal breast or uterus, J. Clin. Endocrin. Metab. 59: 564 (1984).
T. Ikeda, O. Danielpour, P. Galle and D.A. Sirbasku, General Methods for the isolation of acetic acid and heat-stable polypeptide growth factors for mammary and pituitary cells, in: “Methods for Serum-Free Culture of Cells of the Endocrine System,” D. Barnes, D. Sirbasku and G.H. Sato, Alan R. Liss, New York, 1984, p.217.
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Kidwell, W.R., Salomon, D.S., Mohanam, S., Bell, G.I. (1987). Production of Growth Factors by Normal Human Mammary Cells in Culture. In: Goldman, A.S., Atkinson, S.A., Hanson, L.Å. (eds) Human Lactation 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0837-7_25
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DOI: https://doi.org/10.1007/978-1-4899-0837-7_25
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