Stable Inheritance of Bacterial Plasmids: Practical Considerations in the Release of Organisms into the Environment
Most current applications of biotechnology involve the use of genetic engineering for the production of biologically active proteins in cells grown in fermenters. When such products are made in bacterial cells, gene constructs that yield large quantities of the desired product are employed using multicopy bacterial plasmid replicons. The continuing presence in the cell of a plasmid carrying the desired genes commonly can be assured by the addition of a selectable gene to the plasmid replicon and by growth under selective conditions. For fermentations of short duration, continued selection for the presence of the plasmid during the fermentation usually is not required.
KeywordsBacterial Plasmid Fellowship Award Replication Region Stable Inheritance High Copy Number Plasmid
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