Fluidity of Plasmid DNA Fragments Involved in Biodegradation of Aromatic Hydrocarbons by pseudomonas putida Strain R5-3

  • J. V. Leary
  • B. Carney
  • D. D. Focht
Part of the Basic Life Sciences book series (BLSC, volume 45)

Abstract

Pseudomonas putida strain R5-3 isolated from sewage sludge contained two plasmids 100 and 80 kb in size after growth on media containing 4-methylbenzoate (4-MB) as sole carbon source. When transferred to toluene (Tol) or 3-xylene (3-Xyl) as the sole carbon source, the plasmid profile changed with loss of the 80-kb plasmid and the appearance of two plasmids 50 and 60 kb in size. When R5-3 was transferred back to 4-MB from either Tol or 3-Xyl, the original 100- and 80-kb plasmids were present and the 50- and 60-kb plasmids were lost. Restriction enzyme analysis of total plasmid DNA indicated rearrangements and loss of DNA restriction fragments in the strains maintained on Tol and 3-Xyl when compared to the original R5-3 maintained on 4-MB. In the isolate which “retrieved” the original plasmid profile, the restriction enzyme fragment pattern was identical to the original R5-3, in that the fragments which were missing after prolonged growth on Tol or 3-Xyl were again present. The possibility that selected fragments are sequestered in the chromosome is presently under investigation.

Keywords

Sewage Sludge Sole Carbon Source Petroleum Hydrocarbon Pseudomonas Putida Restriction Enzyme Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. V. Leary
    • 1
  • B. Carney
    • 1
  • D. D. Focht
    • 1
  1. 1.University of CaliforniaRiversideUSA

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