A New Method for Bacterial Strain Construction in Biodegradation of Xenobiotic Compounds

  • L. Krockel
  • D. D. Focht
Part of the Basic Life Sciences book series (BLSC, volume 45)

Abstract

Separate continuous cultures of Pseudomonas putida R5-3 (benzene+, toluene+, xylene+, benzoate+), grown on toluene, and Pseudomonas alcaligenes C-O (benzoate+, 3-chlorobenzoate+), grown on benzoate, were concentrated and continuously amalgamated on a ceramic bead-column, which was subjected to a continuous stream of chlorobenzene vapors. A recombinant strain of P. putida CB1-9, able to grow on chlorobenzene, 1, 4-dichlorobenzene (1,4-DCB), and 3-chlorobenzoate, was isolated in less than one month. Chloride was released in stoichiometric amounts when P. putida CB1-9 was grown on either chlorobenzene or 1,4-DCB. Chlorobenzeneutilizing strains were not found in continuous cultures run at the lowest dilution rate (0.05 hr−1) or in the absence of the donor strain, P. alcaligenes C-0. The recombinant strain was related to P. putida R5-3, phenotypically and genetically. The recombinant, however, lost its ability to grow on p- and m-xylenes and the corresponding methylbenzoates. P. putida R5-3 carries a nonconjugative XYL-like plasmid with induction patterns similar to the TOL plasmid pWWO. P. putida CB1-9 carries a 33-kb plasmid which is derived from the 57-kb plasmid of P. putida R5-3, probably by deletion of genes coding for key enzymes in xylene and toluate catabolism.

Keywords

Pseudomonas Putida Methane Formation Anaerobic Biodegradation Lower Dilution Rate Dechlorination Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • L. Krockel
    • 1
  • D. D. Focht
    • 1
  1. 1.University of CaliforniaRiversideUSA

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