Microbial Degradation of Chlorinated Unsaturated Fatty Acids

  • D. Kohler-Staub
  • D. D. Focht
Part of the Basic Life Sciences book series (BLSC, volume 45)

Abstract

Incomplete degradation of multiple halogenated aromatic compounds [e.g., polychlorinated biphenyls (PCBs)] through the meta-fission pathway may be due to accumulation of halogenated unsaturated fatty acids. In order to study their further degradation, the enrichment culture technique was applied to isolate a microorganism capable of growing on the model compound chlorocrotonic acid (3-chlorobutenoic acid). The isolated strain, called CC1, grows on cis-chlorocrotonic acid, trans-chlorocrotonic acid, 3-chlorobutanoic acid, 2-chlorobutanoic acid, 2-chloropropionic acid, and chloracetic acid as sole sources of energy and carbon. Experiments with both resting cells and cell-free extract assays revealed that CC1 produces constitutively a 2-chloroalcanoic acid dehalogenase which acts on mono- and dichloroacetate, 2-chloropropionate, 2, 2-dichloropropionate, and 2-chlorobutanate. The specific activities in terms of chloride release that were measured with cell-free extracts were highest for 2-chloropropionate and 2-chloroacetate—ranging from 0.6 to 1.6 μMol/min/mg protein. Dehalogenase activity on β-chloro-C4 acids was not observed from cell-free extracts of cells grown on chlorinated or nonchlorinated acids, whereas it was observed from whole cells.

Keywords

Microbial Degradation Unsaturated Fatty Acid Aromatic Compound Sole Source Trans Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • D. Kohler-Staub
    • 1
  • D. D. Focht
    • 1
  1. 1.University of CaliforniaRiversideUSA

Personalised recommendations