Advertisement

The Molecular Microbial Ecology of a Catabolic Genotype in a Biological Treatment Process

  • J. W. Blackburn
  • T. L. Donaldson
  • R. K. Jain
  • G. S. Sayler
Part of the Basic Life Sciences book series (BLSC, volume 45)

Abstract

Gene probe technology has been used in conjunction with conventional biochemical assays to characterize the in situ dynamic structure of a microbial ecosystem containing a critical subpopulation that degrades naphthalene. A continuous, lab-scale, activated sludge system fed with a real industrial waste and inoculated with real plant sludge was used. Naphthalene was only 0.2% to 0.06% of the dissolved organic carbon in the industrial feed stream, and the naphthalene-positive genotype (cells containing the NAH7 plasmid) ranged from 0.007% to 0.6% of the total cells (measured by acridine orange direct counts).

Keywords

Biological Treatment Acridine Orange Feed Stream Wood Treatment Wood Preservative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. W. Blackburn
    • 1
  • T. L. Donaldson
    • 2
  • R. K. Jain
    • 1
  • G. S. Sayler
    • 1
  1. 1.The University of TennesseeKnoxvilleUSA
  2. 2.Oak Ridge National LaboratoryOak RidgeUSA

Personalised recommendations