A Shuttle System for the Rapid Introduction of Tracking and other Genes into the Chromosomes of Soil Bacteria

  • Gerald F. Barry
Part of the Basic Life Sciences book series (BLSC, volume 45)

Abstract

The ability to easily and accurately track genetically engineered bacteria is an important aspect of their introduction into the environment. The genes introduced into these bacteria should also be inserted in such a way that their likelihood of loss or transfer to other bacteria is minimized. These two aims have been addressed for the fluorescent Pseudomonads by development of the Tn-derivative (Tn7-lac) gene insertion system that inserts the termini of the transposon but not those genes required for transposition. The genes (lacZY) that provide the phenotype used to select the recombinant derivatives also provide a phenotype that can be used very effectively in the tracking of these recombinant bacteria even in the presence of high numbers of similar indigenous bacteria. In the system above, the addition of other genes to the element (and then to the bacteria) was difficult, as was the introduction of plasmids of different incompatibilities into many target bacteria.

Keywords

Microbial Biomass Fluorescent Pseudomonad Target Bacterium Recombinant Bacterium Delivery Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Gerald F. Barry
    • 1
  1. 1.Plant Microbiology Group, Biological Sciences DepartmentMonsanto Company AA2GSt. LouisUSA

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