The Ecology of an Anaerobic Dechlorinating Consortium

  • James M. Tiedje
  • Todd O. Stevens
Part of the Basic Life Sciences book series (BLSC, volume 45)


The mechanisms used by anaerobic bacterial communities to degrade organic molecules are of interest not only to increase our understanding of biogeochemical cycling, but also to explore the potential of microorganisms to degrade xenobiotic molecules in anaerobic environments (12). This has been of increasing concern in recent years, as many man-made chemicals are found to be toxic or mutagenic. New methods must be found to dispose of these compounds when they are no longer of use and to clean up contaminated areas. Digestion by anaerobic bacteria is an attractive possibility for disposal of synthetic chemicals, because it can require less energy than other methods of waste disposal; construction costs for anaerobic treatment facilities are lower; and anaerobic digestion offers the possibility of recovering useful products, such as intermediary metabolites or methane gas (30). Studying these processes also aids in predicting the fate of xenobiotics that have been introduced into natural anaerobic envi-ronments.


Sewage Sludge Anaerobic Digestion Sodium Dithionite Rumen Fluid Anaerobic Biodegradation 
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  1. 1.
    Alexander, M. (1973) Nonbiodegradable and other recalcitrant molecules. Biotechnol. Bioengineer. 15:611–647.CrossRefGoogle Scholar
  2. 2.
    Alexander, M.A (1979) Recalcitrant molecules, fallible microorganisms. In Microbial Ecology: A Conceptual Approach, J.M. Lynch and N.J. Poole, eds. Blackwell Scientific Publications, Oxford.CrossRefGoogle Scholar
  3. 3.
    Balch, W.E., G.E. Fox, L.J. Magrum, C.R. Woese, and R.S. Wolfe (1979) Methanogens: Reevaluation of a unique biological group. Microbiol. Rev. 43:160–296.Google Scholar
  4. 4.
    Beaty, P.S., N.Q. Wofford, and M.J. McInerney (1987) Separation of Syntrophomonas wolfei from Methanospirillum hungatei in syntrophic cocultures using Percoll gradients. Appl. Environ. Microbiol. 53:1183–1185.PubMedGoogle Scholar
  5. 5.
    Boyd, S.A., and D.R. Shelton (1984) Anaerobic biodegradation of chlorophenols in fresh and acclimated sludge. Appl. Environ. Microbiol. 47:272–277.PubMedGoogle Scholar
  6. 6.
    Bryant, M.P., E.A. Wolin, M.J. Wolin, and R.W. Wolfe (1967) Methanobacillus omelianskii, a symbiotic association of two species of bacteria. Archiv für Mikrobiol. 59:20–31.CrossRefGoogle Scholar
  7. 7.
    Crow, W.D., R. Machanoff, and H.I. Adler (1985) Isolation of anaerobes using an oxygen reducing membrane fraction: Experiments with acetone butanol producing organisms. J. Microbiol. Meth. 4:133–139.CrossRefGoogle Scholar
  8. 8.
    DeWeerd, K.A., J.M. Suflita, T.G. Linkfield, J.M. Tiedje, and P.H. Pritchard (1986) The relationship between reductive dehalogenation and other aryl substituent removal reactions catalyzed by anaerobes. FEMS Microbiol. Ecol. 38:331–339.CrossRefGoogle Scholar
  9. 9.
    Dolfing, J., and J.M. Tiedje (1986) Hydrogen cycling in a three-tiered food web growing on the methanogenic conversion of 3-chlorobenzoate. FEMS Microbiol. Ecol. 38:293–298.CrossRefGoogle Scholar
  10. 10.
    Dolfing, J., and J.M. Tiedje (1987) Growth yield increase linked to reductive dechlorination in a defined 3-chlorobenzoate degrading methanogenic coculture. Arch. Microbiol. (in press).Google Scholar
  11. 11.
    Fathepure, B.Z., J.M. Tiedje, and S.A. Boyd (1987) Reductive dechlorination of 4-chlororesorcinol by anaerobic microorganisms. Environ. Toxicol. Chem. Vol. 6 (in press).Google Scholar
  12. 12.
    Fathepure, B.Z., J.M Tiedje, and S.A. Boyd (1988) Reductive dechlorination of hexachlorobenzene to tri-and dichlorobenzenes in anaerobic sewage sludge. Appl. Environ. Microbiol. (in press).Google Scholar
  13. 13.
    Gschwend, P.M., J.K. MacFarlane, and K.A. Newman (1985) Volatile halogenated organic compounds released to seawater from temperate marine macroalgae. Science 227:1033–1035.PubMedCrossRefGoogle Scholar
  14. 14.
    Hamilton, W.A. (1979) Microbial energetics and metabolism. In Microbial Ecology: A Conceptual Approach, J.M. Lynch and N.J. Poole, eds. Blackwell Scientific Publications, Oxford.Google Scholar
  15. 15.
    Hobson, P.N., S. Bousfield, and R. Summers (1974) Anaerobic digestion of organic matter. Crit. Rev. Environ. Control 4:131–191.CrossRefGoogle Scholar
  16. 16.
    King, G.M. (1986) Inhibition of microbial activity in marine sediments by a bromophenol from a hemichordate. Nature 323:257–259.CrossRefGoogle Scholar
  17. 17.
    Lovely, D.R., and M.J. Klug (1982) Intermediary metabolism of organic matter in the sediments of a eutrophic lake. Appl. Environ. Microbiol. 43:552–560.Google Scholar
  18. 18.
    Mikeseil, M.D., and S.A. Boyd (1985) Reductive dechlorination of the pesticides 2,4-D, 2,4,5-T and pentachlorophenol in anaerobic sludges. J. Environ. Qual. 14:337–340.CrossRefGoogle Scholar
  19. 19.
    Mikeseil, M.D., and S.A. Boyd (1986) Complete reductive dechlorination and mineralization of pentachlorophenol by anaerobic microorganisms. Appl. Environ. Microbiol. 52:861–865.Google Scholar
  20. 20.
    Postgate, J.R. (1979) The Sulphate Reducing Bacteria, Cambridge University Press, Cambridge.Google Scholar
  21. 21.
    Sato, T., M. Mukaida, Y. Ose, H. Nagase, and T. Ishikawa (1985) Mutagenicity of chlorinated products from soil humic substances. The Science of the Total Environment 46:229–241.PubMedCrossRefGoogle Scholar
  22. 22.
    Shelton, D.R., and J.M. Tiedje (1984) General method for determining anaerobic biodegradation potential. Appl. Environ. Microbiol. 47:850–857.PubMedGoogle Scholar
  23. 23.
    Shelton, D.R., and J.M. Tiedje (1984) Isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3-chlorobenzoic acid. Appl. Environ. Microbiol. 48:840–848.PubMedGoogle Scholar
  24. 24.
    Sleat, R., and J.P. Robinson (1984) The bacteriology of anaerobic degradation of aromatic compounds. J. Appl. Bacteriol. 57:381–394.PubMedCrossRefGoogle Scholar
  25. 25.
    Stevens, T.O., and J.M. Tiedje (1988) Carbon dioxide fixation and mixotrophic metabolism by strain DCB-1, a unique sulfidogenic dehalogenating organism. Appl. Environ. Microbiol. (submitted for publication).Google Scholar
  26. 26.
    Stevens, T.O., T.G. Linkfield, and J.M. Tiedje (1988) Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium. Appl. Environ. Microbiol. (submitted for publication).Google Scholar
  27. 27.
    Suflita, J.M., J. Robinson, and J.M. Tiedje (1983) Kinetics of microbial dehalogenation of haloaromatic substrates in methanogenic environments. Appl. Environ. Microbiol. 45:1466–1473.PubMedGoogle Scholar
  28. 28.
    Suflita, J M., J. Stout, and J.M. Tiedje (1984) Dechlorination of (2,4,5-trichlorophenoxy) acetic acid by anaerobic microorganisms. J. Agric. Food Chem. 32:218–221.CrossRefGoogle Scholar
  29. 29.
    Suflita, J.M., A. Horowitz, D.R. Shelton, and J.M. Tiedje (1982) Dehalogenation: A novel pathway for the anaerobic biodegradation of haloaromatic compounds. Science 218:1115–1117.PubMedCrossRefGoogle Scholar
  30. 30.
    Tiedje, J.M., S.A. Boyd, and B.Z. Fathepure (1987) Anaerobic degradation of chlorinated aromatic hydrocarbons. Dev. Indus. Microbiol. 27:117–127.Google Scholar
  31. 31.
    Tiedje, J.M., S.A. Boyd, and J.F. Quensen III (1987) Reductive dechlorination of PCBs in anaerobic microbial communities. In Research and Development Program for the Destruction of PCBs, H.L. Finkbeiner and S.B. Hamilton, eds. General Electric Company Corporate Research and Development, Sixth Progress Report, Schenectady, New York, pp. 29–33.Google Scholar
  32. 32.
    Wolin, M.J. (1979) The rumen fermentation: A model for microbial interactions in anaerobic ecosystems. Adv. Microb. Ecol. 3:49–77.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • James M. Tiedje
    • 1
  • Todd O. Stevens
    • 1
  1. 1.Departments of Microbiology and Public Health and of Crop and Soil SciencesMichigan State UniversityEast LansingUSA

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