Single Crystals of the Photochemical Reaction Center from Rhodobacter sphaeroides Wild Type Strain 2.4.1 Analyzed by Polarized Light

  • Harry A. Frank
  • Shahriar S. Taremi
  • James R. Knox
  • Werner Mäntele
Part of the NATO ASI Series book series (NSSA, volume 149)


The crystal structures of the reaction centers from the carotenoidless mutant Rb. sphaeroides R26 and from Rps. viridis, have provided the means to explore the relationships between the structures of the chromophores and their spectroscopic properties (Deisenhofer et al., 1985; Allen et al., 1986; Chang et al., 1986). Prompted by the fact that one specific relationship, that between the carotenoid molecule and its various spectroscopic properties has not been fully elucidated by the existing structures, we set out to crystallize and study the reaction center protein from Rb. sphaeroides 2.4.1. The abundance of biochemical, spectroscopic and dynamics information previously obtained (Cogdell et al., 1976; Parson & Monger, 1976; Lutz et al., 1978; Vermeglio et al., 1978; McGann & Frank, 1985; Chadwick & Frank, 1986; Gagliano et al., 1986) for this particular bacterial strain and its associated carotenoid, spheroidene, makes the elucidation of the structure of this reaction center complex of particular importance.


Reaction Center Wild Type Strain Primary Donor Transition Moment Crystal Axis 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Harry A. Frank
    • 1
  • Shahriar S. Taremi
    • 1
  • James R. Knox
    • 2
  • Werner Mäntele
    • 3
  1. 1.Departments of ChemistryUniversity of ConnecticutStorrsUSA
  2. 2.Molecular and Cell BiologyUniversity of ConnecticutStorrsUSA
  3. 3.Institut für Biophysik und StrahlenbiologieFreiburgGermany

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