Abstract
Detailed theories of electron transfer in reaction centers (RCs) require knowledge of their three dimensional structure. We have determined the structure of RCs from Rb. sphaeroides by x-ray diffraction of single crystals. Diffraction data of RCs from both the carotenoidless mutant, R-26, and the wild type strain, 2.4.1 were analyzed at resolutions of 2.8 Å and 3.5 Å respectively. These structures have been refined to current R-factors of 25% (for the R26 data) and 22% (for the 2.4.1. data). Details concerning data collection and analysis, with descriptions of the structure of the RC from the R-26 strain have been presented elsewhere (1–4). In this report we shall focus on the general features of the structure and compare them with those reported for the RC from R. viridis (5). The structures from these two species have been shown by the molecular replacement method to be homologous (6,7). We shall emphasize the importance of the structural features to the function of electron transfer.
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References
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Allen, J.P., Feher, G., Yeates, T.O., Komiya, H., Rees, D.C. (1988). Structure of the Reaction Center from Rhodobacter sphaeroides R-26 and 2.4.1. In: Breton, J., Verméglio, A. (eds) The Photosynthetic Bacterial Reaction Center. NATO ASI Series, vol 149. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0815-5_2
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DOI: https://doi.org/10.1007/978-1-4899-0815-5_2
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