Abstract
At the predecessor of this conference in Feldafing (1985) great interest in the Stark effect in photosynthetic reaction centers (RCs) was expressed [1]. At that time only an abstract [2] had been published showing that the bacteriochlorophyll dimer (BChl2; the primary donor) had a large Stark effect compared to the other (accessory) chromophores. In the intervening period, several groups have contributed to this topic [3–5]. In this report we present detailed spectra and quantitative data on RCs from several organisms, and compare the results with those obtained by other groups and with theoretical predictions. Since much of the work has already been published, we shall endeavor to minimize duplication and focus on newer developments, qualitative and quantitative discussions, and prospects for the future.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
J. Jortner and M. E. Michel-Beyerle, Some aspects of energy transfer in antennas and electron transfer in reaction centers of photosynthetic bacteria, in: “Antennas and Reaction Centers of Photosynthetic Bacteria,” M. E. Michel-Beyerle, ed. Springer, Berlin, pp. 345–366 (1985).
D. deLeeuw, M. Malley, G. Buttermann, M. Y. Okamura, and G. Feher, The Stark effect in reaction centers from Rhodopseudomonas sphaeroides, Biophys. J. (Abstr.), 111a (1982).
D. J. Lockhart and S. G. Boxer, Magnitude and direction of the change in dipole moment associated with excitation of the primary electron donor in Rhodopseudomonas sphaeroides reaction centers, Biochemistry 26: 664–668 (1987). (Correction in Biochemistry 26:2958 (1987)).
M. Lösche, G. Feher and M. Y. Okamura, The Stark effect in reaction centers from Rhodobacter sphaeroides R-26 and Rhodopseudomonas viridis, Proc. Natl. Acad. Sci. USA 84:7537–7541 (1987).
H. P. Braun, M. E. Michel-Beyerle, J. Breton, S. Buchanan and H. Michel, Electric field effect on absorption spectra of reaction centers of Rb. sphaeroides and Rps. viridis, FEBS Lett. 221: 221–225 (1987).
W. Liptay, Dipole moments and polarizabilities of molecules in excited electronic states, in “Excited States”, E. C. Lim, ed., Academic Press, Inc., New York, pp. 129–229 (1974).
R. Reich and S. Schmidt, Uber den Einfluss elektrischer Felder auf das Absorptionsspektrum von Farbstoffmolekülen in Lipidschichten. I. Theorie, Ber. Bunsenges. Phys. Chem. 76: 589–598 (1972).
R. Reich, Intrinsic probes of charge separation, in “Light-Induced Charge Separation in Biology and Chemistry”, H. Gerischer and J. J. Katz, eds., Verlag Chemie, Weinheim (West Germany), pp. 361–387 (1979).
C. J. F. Böttcher, “Theory of Electric Polarization”, Elsevier, New York, pp. 159–204 (1973).
M. Malley, G. Feher and D. Mauzerall, The Stark effect in porphyrins, J. Mol. Spectrosc. 25: 544–548 (1968).
O. Nanba and K. Satoh, Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559, Proc. Natl. Acad. Sci. USA 84: 109–112 (1987).
M. Y. Okamura, K. Satoh, R. A. Isaacson, and G. Feher, Evidence of the primary charge separation in the D1 D2 complex of photosystem II from spinach: EPR of the triplet state, in: “Progress in Photosynthesis Research,” Vol. I, pp. 379–381, J. Biggins, ed., Martinus Nijhoff, Boston (1986).
M. Lösche, K. Satoh, G. Feher, and M.Y. Okamura, Stark effect in PSII from spinach, Biophys. J. (Abstract), February 1988, in press.
J. C. Williams, L. A. Steiner, and G. Feher, Primary Structure of the Reaction Center From Rhodopseudomonas sphaeroides, Proteins 1:312–325 (1986).
R. A. Marcus, Superexchange versus an intermediate BChl− mechanism in reaction centers of photosynthetic bacteria, Chem. Phys. Lett. 113: 471–477 (1987).
M. E. Michel-Beyerle, M. Plato, J. Deisenhofer, H. Michel, M. Bixon and J. Jortner, Unidirectionality of charge separation in reaction centers of photosynthetic bacteria, Biochim. Biophys. Acta, in press.
P. O. J. Scherer and S. F. Fischer, On the Stark effect for bacterial photosynthetic reaction centers, Chem. Phys. Lett. 131: 153–159 (1986).
A. Ogrodnik, N. Remy-Richter, M. E. Michel-Beyerle and R. Feick, Observation of activationless recombination in reaction centers of R. sphaeroides. A new key to the primary electron-transfer mechanism, Chem. Phys. Letters 135: 576–581 (1987).
R. A. Marcus, Electron transfer and the bacterial photosynthetic reaction centers; in these Proceedings.
S. F. Fischer and P. O. J. Scherer, Analysis of different mechanisms for the initial charge separation within the reaction center Rps. viridis; in these Proceedings.
W. W. Parson and A. Warshel, Calculations of electronic interactions in reaction centers; in these Proceedings.
W. W. Parson, N. W. T. Woodbury, M. Becker, C. Kirmaier and D. Holten, Kinetics and mechanisms of initial electron-transfer reactions in Rhodopseudomonas sphaeroides reaction centers, in: “Antennas and Reaction Centers of Photosynthetic Bacteria,” M. E. Michel-Beyerle, ed. Springer, Berlin, pp. 278–285.
R. S. Mulliken and W. B. Person, “Molecular Complexes”, Wiley-Interscience, New York, pp. 9–22 (1969).
J. P. Allen, G. Feher, T. O. Yeates, H. Komiya and D. C. Rees, Structure of the reaction center from Rhodobacter sphaeroides R-26: The cofactors, Proc. Natl. Acad. Sci. USA 84: 5730–5734 (1987).
H. Michel, O. Epp and J. Deisenhofer, Pigment-protein interactions in the photosynthetic reaction centers from Rhodopseudomonas viridis, EMBO J. 5: 2445–2451 (1986).
W. W. Parson and A. Warshel, Spectroscopic properties of photosynthetic reaction centers. II. Application of the theory to Rhodopseudomonas viridis, J. Am. Chem. Soc. 109: 6152–6163 (1987).
M. Plato, personal communication.
P. Jursinic and Govindjee, Temperature dependence of delayed light emission in the 6 to 340 microsecond range after a single flash in chloroplasts, Photochem. Photobiol. 26:617–628 (1977).
A. V. Shuvalov, A. V. Klevanik, A. V. Sharkov, Ju. A. Matveetz, and P. G. Krukov, Picosecond detection of BChl-800 as an intermediate electron carrier between selectively excited P870 and bacteriopheophytin in Rhodospirillum rubrum reaction centers, FEBS Lett. 91:135-139.
N. W. Woodbury, M. Becker, D. Middendorf and W. W. Parson, Picosecond kinetics of the initial photochemical electron-transfer reaction in bacterial photosynthetic reaction centers, Biochemistry 24: 7516–7521 (1985).
J. L. Martin, J. Breton, A. J. Hoff, A. Migus and A. Antonetti, Femtosecond spectroscopy of electron transfer in the reaction center of the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26: Direct electron transfer from the dimeric bacteriochlorophyll primary donor to the bacteriopheophytin acceptor with a time constant of 2.8 ± 0.2 psec, Proc. Natl. Acad. Sci. USA 83: 957–961 (1986).
J. Breton, J. L. Martin, A. Migus, A. Antonetti and A. Orszag, Femtosecond spectroscopy of excitation energy transfer and initial charge separation in the reaction center of the photosynthetic bacterium Rhodopseudomonas midis, Proc. Natl. Acad. Sci. USA 83: 5121–5125 (1986).
A. Gopher, Y. Blatt, M. Schönfeld, M. Y. Okamura, G. Feher, and M. Montai, The effect of an applied field on the charge recombination kinetics in reaction centers reconstituted in planar lipid bilayers, Biophys. J. 48: 311–320 (1985).
Z. D. Popovic, G. J. Kovacs, P. S. Vincent, G. Alegria, and P. L. Dutton, Electric field dependence of recombination kinetics in reaction centers of photosynthetic bacteria, Chem. Phys. 110: 227–237 (1986).
T. Arno, A. Gopher, M. Y. Okamura, and G. Feher, Dependence of the recombination rate D+Q− A → DQA on the electric field applied to reaction centers from Rb. sphaeroides R-26 incorporated into a planar lipid bilayer, Biophys. J. (Abstract), February 1988, in press.
G. Feher, T. Arno, and M. Y. Okamura, The effect of an electric field on the charge recombination rate of D+Q→ A → DQA in reaction centers from Rhodobacter sphaeroides R-26; in these Proceedings.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lösche, M., Feher, G., Okamura, M.Y. (1988). The Stark Effect in Photosynthetic Reaction Centers from Rhodobacter sphaeroides R-26, Rhodopseudomonas viridis and the D1D2 Complex of Photosystem II from Spinach. In: Breton, J., Verméglio, A. (eds) The Photosynthetic Bacterial Reaction Center. NATO ASI Series, vol 149. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0815-5_17
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0815-5_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0817-9
Online ISBN: 978-1-4899-0815-5
eBook Packages: Springer Book Archive