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The Crystal Structure of the Photosynthetic Reaction Center from Rhodopseudomonas viridis

  • Johann Deisenhofer
  • Hartmut Michel
Chapter
Part of the NATO ASI Series book series (NSSA, volume 149)

Abstract

The photosynthetic reaction center (RC) from Rhodopseudomonas viridis (R. viridis) was one of the first integral membrane proteins which could be crystallized (Michel, 1982). The crystals turned out to be suitable for X-ray structure analysis at atomic resolution. In a study at 3A resolution, using phases determined from multiple isomorphous replacement experiments with heavy atom compounds, the arrangement of the major prosthetic groups (Deisenhofer et al., 1984), and the folding of the protein subunits (Deisenhofer et al., 1985) were determined. Sequencing of the RC’s protein subunits (Michel et al., 1985; Michel et al., 1986a; Weyer et al., 1987) facilitated interpretation of the electron density map and model building; information from X-ray diffraction and from sequencing together allowed a detailed description of pigment-protein interactions in the RC (Michel et al., 1986b).

Keywords

Photosynthetic Reaction Centre Bind Water Molecule Membrane Protein Complex Primary Charge Separation Membrane Span Helix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Deisenhofer, J., Epp, O., Miki, K., Huber, R. and Michel, H. (1985). Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3 A resolution, Nature 318, 618–624.PubMedCrossRefGoogle Scholar
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  5. Michel, H., Weyer, K.A., Gruenberg, H., Dunger, I., Oesterhelt, D. and Lottspeich, F. (1986a). The ‘light’ and ‘medium’. subunits of the photosynthetic reaction centre from Rhodopseudomonas viridis: isolation of the genes, nucleotide and amino acid sequence, EMBO J. 5, 1149–1158.PubMedGoogle Scholar
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  7. Weyer, K.A., Lottspeich, F., Gruenberg, H., Lang, F., Oesterhelt, D. and Michel, H. (1987). Amino acid sequence of the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis, EMBO J. 6, 2197–2202.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Johann Deisenhofer
    • 1
  • Hartmut Michel
    • 1
  1. 1.Max-Planck-Institut fuer BiochemieMartinsriedGermany

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