Substance P- and Somatostatin-Like Immunoreactivity in Avian Encapsulated Mechanoreceptors

  • C. Chouchkov
  • M. Davidoff

Abstract

The identity of neurotransmitters utilized by primary sensory neurons has not been firmly established, although several candidates for neurotransmitters or modulators have been suggested (Salt and Hill, 1983). In particular, many attempts to prove histochemically any of the well-known neurotransmitters or modulators in sensory receptors have been unsuccessful. Moreover, the well documented distribution of neuropeptides in some somata of primary sensory ganglia (substance P (SP) — Katz and Karten, 1980; somatostatin (Som) — Kummer and Heym, 1986; vasoactive intestinal polypeptide (VIP) — Skofitsch et al., 1985; cholecystokinin (CCK) — Kuwayama and Stone, 1986; opioid peptides — Weihe et al., 1985) has not been generally confirmed in corresponding sensory nerve fibers of encapsulated or non-capsulated receptors. Some efforts have been made to localize in the light microscope SP-LI (like-immunoreactive) nerve fibers in human digital skin including the sensory nerves entering Meissner corpuscles (Dalsgaard et al., 1983). On the other hand, Hartschuh et al. (1979) visualized immunohistochemically a met-enkephalin-LI in Merkel cells. Recently, Björklund et al. (1986) found SP- and neurokinin A-LI only in sensory nerve fibers, whereas VIP-LI has been observed predominantly in autonomic nerves but also in some sensory nerve fibers. All known available immunohistochemical investigations examine the above mentioned neuropeptides at the light microscopical level. The aim of the present communication is to extend the knowledge concerning the existence of new peptides in bird mechanoreceptors and to define their precise cytological localization at the electron microscopical level.

Keywords

Nerve Fiber Vasoactive Intestinal Polypeptide Sensory Receptor Primary Antiserum Trigeminal Neuron 
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References

  1. Björklund, H., Dalsgaard, C.-J., Jonsson, C.-E., and Hermansson, A., 1986, Sensory and autonomic innervation of non-hairy and hairy human skin. An immunohistochemical study, Cell Tissue Res., 243, 51–57.PubMedCrossRefGoogle Scholar
  2. Chouchkov, C., 1978, Cutaneous receptors, Adv. Anat. Embr. Cell Biol., 54, 1–62.Google Scholar
  3. Dalsgaard, C.-J., Jonsson, C.-E., Hökfelt, T., and Cuello, A.C., 1983, Localization of substance P-immunoreactive nerve fibers in the human digital skin, Experientia, 39, 1018–1020.PubMedCrossRefGoogle Scholar
  4. Hartschuh, W., Weihe, E., Buchler, M., Helmstaedter, V., Feurle, G.E., and Forssmann, W.G., 1979, Met-enkephalinlike immunoreactivity in Merkel cell, Cell Tissue Res., 201, 343–348.PubMedCrossRefGoogle Scholar
  5. Idé, C., and Munger, B.L., 1978, A cytologic study of Grandry corpuscle development in chicken tow skin, J. Comp. Neurol., 179, 301–324.PubMedCrossRefGoogle Scholar
  6. Katz, D.M., and Karten, H.J., 1980, Substance P in the vagal sensory ganglia: localization in cell bodies and pericellular arborizations, J. Comp. Neurol., 193, 549–564.PubMedCrossRefGoogle Scholar
  7. Kummer, W., and Heym C., 1986, Correlation of neuronal size and peptide immunoreactivity in the quinea-pig trigeminal ganglion, Cell Tissue Res., 245, 657–665.PubMedCrossRefGoogle Scholar
  8. Kuwayama, Y., and Stone, R.A., 1986, Neuropeptide immunoreactivity of pericellular baskets in the guinea-pig trigeminal ganglion, Neurosci. Lett., 64, 169–172.PubMedCrossRefGoogle Scholar
  9. Páč, L., and Malinovsky, L., 1985, Development of the Grandry corpuscles in the skin of the beak of the domestic duck, Folia Morphologica, 33, 379–384.PubMedGoogle Scholar
  10. Salt, T.E., and Hill, R.G., 1983, Neurotransmitter candidates of somatosensory primary afferent fibres, Neuroscience, 10, 1083–1103.PubMedCrossRefGoogle Scholar
  11. Saxod, R., 1978, Development of cutaneous sensory receptors in birds, in: Handbook of Sensory Physiology, vol.9, M. Jacobson, ed., Springer Verlag, Berlin-Heidelberg-New York.Google Scholar
  12. Skofitsch, G., Zamir, N., Heike, C.J., Savitt, J.M., and Jacobowitz, D.M., 1985, Corticotropin releasing factor-like immunoreactivity in sensory ganglion and capsaicin sensitive neurons of the rat central nervous system: co-localization with other neuropeptides, Peptides, 6, 307–318.PubMedCrossRefGoogle Scholar
  13. Sternberger, L.A., Hardy, P.H., Curculis, J.J., and Meyer, H.G., 1970, The unlabelled antibody enzyme method of immunohistochemistry, J. Histochem. Cytochem., 18, 315–333.PubMedCrossRefGoogle Scholar
  14. Weihe, E., Hartschuh, W., and Weber, E., 1985, Prodynorphin opioid peptides in small somatosensory primary afferents of guinea pig, Neurosci. Lett., 58, 347–352.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • C. Chouchkov
    • 1
    • 2
  • M. Davidoff
    • 1
    • 2
  1. 1.Department of AnatomyHigher Medical InstituteStara ZagoraBulgaria
  2. 2.Regeneration Research LaboratoryBulgarian Academy of SciencesSofiaBulgaria

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