Regeneration of Muscle Spindles in Grafted Extensor Digitorum Longus Muscle of the Rat
Mammalian skeletal muscles possess considerable capacity to regenerate following muscle injury (e.g. Studitsky, 1977). Grafting of a muscle results in the degeneration of muscle fibers due to ischemia, but within several days, their regeneration takes place and the differentiating graft is revascularized and reinnervated during the first three weeks after the operation (for review see Carlson, 1983). Intrafusal fibers of muscle spindles undergo the same process of degeneration and regeneration as the whole muscle and they do regenerate in free grafts (Carlson and Gutmann, 1975; Schmalbruch, 1977; Rogers, 1982). The standard technique of free grafting has a great drawback, as the onset and extent of regeneration of the neuromuscular pathways disrupted at the time of grafting is difficult to control. To overcome this disadvantage, nerve-intact grafts (Rogers and Carlson, 1981), bupivacaine treatment (Milburn, 1976) or other techniques, e.g. temporary ischemia (Diwan and Milburn, 1986; Scott et al., 1988) were introduced for the study of spindle regeneration. These techniques leave the innervation pathways in the muscle intact, thus giving a better chance for regeneration than can be expected after standard grafting, where the reinnervation is likely to be more random. Because of the possible practical utilization, we returned to the classical model of standard free grafting, but we allowed a sufficiently long time for muscle regeneration. In the present paper we focussed on the histochemical reactions of intrafusal fibers after autotransplantation. The preliminary results were published previously (Soukup, 1981).
KeywordsMuscle Spindle Mammalian Skeletal Muscle Free Graft Muscle Spin Intrafusal Muscle
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