In Vitro Analysis of Plasma Protein Diffusion in Crosslinked Gelatin Coatings Used for Blood Pumps

  • Hideto Emoto
  • Helen Kambic
  • Hiroaki Harasaki
  • Yukihiko Nosè


Glutaraldehyde crosslinked gelatin surfaces, used as blood pump coatings in calves, have shown excellent long term blood compatibility. The study evaluated the diffusion and kinetics of plasma proteins into gelatin gel matrices using SDS-PAGE and GPC (gel permeation chromatography). The effects of plasma protein diffusion within the gel and the reactivity of bovine platelets to this surface were evaluated in vitro. Five percent crosslinked gelatin gels, 30–50 μm thick, were incubated with fresh bovine plasma for intervals of 5 minutes to 24 hours. Platelet reactivity to the gel surface was examined by exposing the gel surfaces to platelet rich plasma for 2 hours. Changes in platelet morphology were observed by SEM.


Plasma Protein Platelet Rich Plasma Platelet Reactivity Blood Pump Cleveland Clinic Foundation 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Hideto Emoto
    • 1
  • Helen Kambic
    • 1
  • Hiroaki Harasaki
    • 1
  • Yukihiko Nosè
    • 1
  1. 1.Department of Artificial OrgansThe Cleveland Clinic Foundation, One Clinic CenterClevelandUSA

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