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In Vitro Analysis of Plasma Protein Diffusion in Crosslinked Gelatin Coatings Used for Blood Pumps

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Progress in Biomedical Polymers

Abstract

Glutaraldehyde crosslinked gelatin surfaces, used as blood pump coatings in calves, have shown excellent long term blood compatibility. The study evaluated the diffusion and kinetics of plasma proteins into gelatin gel matrices using SDS-PAGE and GPC (gel permeation chromatography). The effects of plasma protein diffusion within the gel and the reactivity of bovine platelets to this surface were evaluated in vitro. Five percent crosslinked gelatin gels, 30–50 μm thick, were incubated with fresh bovine plasma for intervals of 5 minutes to 24 hours. Platelet reactivity to the gel surface was examined by exposing the gel surfaces to platelet rich plasma for 2 hours. Changes in platelet morphology were observed by SEM.

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Authors and Affiliations

  1. Department of Artificial Organs, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, Cleveland, Ohio, 44195-5132, USA

    Hideto Emoto, Helen Kambic, Hiroaki Harasaki & Yukihiko Nosè

Authors
  1. Hideto Emoto
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  2. Helen Kambic
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  3. Hiroaki Harasaki
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  4. Yukihiko Nosè
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Editor information

Editors and Affiliations

  1. Department of Chemistry, Youngstown State University, Youngstown, Ohio, USA

    Charles G. Gebelein

  2. Atrix Laboratories, Inc., Fort Collins, Colorado, 80525, USA

    Richard L. Dunn

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© 1990 Springer Science+Business Media New York

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Emoto, H., Kambic, H., Harasaki, H., Nosè, Y. (1990). In Vitro Analysis of Plasma Protein Diffusion in Crosslinked Gelatin Coatings Used for Blood Pumps. In: Gebelein, C.G., Dunn, R.L. (eds) Progress in Biomedical Polymers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0768-4_24

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  • DOI: https://doi.org/10.1007/978-1-4899-0768-4_24

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0770-7

  • Online ISBN: 978-1-4899-0768-4

  • eBook Packages: Springer Book Archive

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