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The Poly(nonapeptide) of Elastin: A New Elastomeric Polypeptide Biomaterial

  • Dan W. Urry
  • John Jaggard
  • R. Dean Harris
  • D. K. Chang
  • Kari U. Prasad

Abstract

This paper reports the first data on a new member of the class of elastomeric polypeptide biomaterials. The new member is poly(VPGFGVGAG). The synthesis is demonstrated by carbon-13 and proton nuclear magnetic resonance spectra; the temperature ranges for the inverse temperature transition have determined by temperature profiles for turbidity formation (aggregation); and the material has been crosslinked by γ-irradiation to produce elastomeric matrices. Poly(VPGFGVGAG) is distinguished from elastomeric poly(VPGVG) and poly(VPGG) by exhibiting a greater elastic modulus for the same cross-linking treatment, some two orders of magnitude larger, and by simultaneously being chemotactic toward endothelial cells and fibroblasts. These properties appear to be particularly appropriate for consideration of this bioelastic as a scaffolding for the reconstruction of ligaments.

Keywords

Benzyl Ester Proton Nuclear Magnetic Resonance Spectrum Nuclear Overhauser Enhancement Spectroscopy Acid Benzyl Ester Viscoelastic Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Boc

tert-butyloxycarbonyl

OBzl

benzyl ester

DMF

dimethylformamide

DMSO

dimethylsulfoxide

EDe

1 ethyl-3-dimethylaminopropyl carbodiimide

HOBt

1-hydroxybenzotriazole

IBCF

isobutylchloroformate

NMM

N-methylmorpholine

ONp

p-nitrophenyl ester

TFA

trifluoroacetic acid

A

alanine

G

glycine

F

phenylalanine

P

proline

V

valine

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Dan W. Urry
    • 1
  • John Jaggard
    • 1
  • R. Dean Harris
    • 1
  • D. K. Chang
    • 1
  • Kari U. Prasad
    • 1
  1. 1.Laboratory of Molecular Biophysics, School of MedicineThe University of Alabama at BirminghamBirminghamUSA

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