Quantification of Steroids via a Polymer Linked Second Antibody Enzyme Immunoassay System: Methods of Linking Anti-Rabbit IgG to Poly(styrene)

  • D. J. Kesler
  • H. Khazali
  • R. J. Favero


Antibodies have been immobilized on polymers since the 1960s and numerous variations have been developed for commercially available immunodiagnostic tests. Several procedures of linking antibodies to polymers, interest in increasing the concentrations of linked antibodies to polymers, and interest in conserving the limited quantities of rare reagent exist. We have developed a partial denaturation procedure of linking anti-rabbit IgG to poly(styrene) that improved our antibody linking by 40 times. Utilization of these anti-rabbit IgG coated tubes in our polymer linked second antibody enzyme immunoassay produced highly specific, sensitive and accurate quantitative analytical procedures of detecting progesterone and testosterone rapidly.


Enzyme Immunoassay Covalent Linkage Testosterone Propionate Testosterone Enanthate Sodium Acetate Solution 
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  1. 1.
    R. S. Yalow, Science, 200, 1236 (1978).CrossRefGoogle Scholar
  2. 2.
    P. Tijssen, “Laboratory Techniques in Biochemistry and Molecular Biology, Practice & Theory of Enzyme Immunoassays,” Elsevier, Amsterdam, 1985.Google Scholar
  3. 3.
    K. Catt & G. W. Tregear, Science, 158, 1570.Google Scholar
  4. 4.
    D. J. Kesler & M. F. Gibson, Proc. IVth Int. Symp. Vet. Lab. Diag., 379 (1986).Google Scholar
  5. 5.
    E. R. Valle & D. J. Kesler, Proc. IVth Symp. Vet. Lab. Diag., 372 (1986).Google Scholar
  6. 6.
    S. W. Chen & D. J. Kesler, J. Anim. Sci., 65 (Suppl. 1), 151 (1987).Google Scholar
  7. 7.
    K. Heide & H. G. Schwick, “Handbook of Experimental Immunology,” Blackwell, Oxford, 1978.Google Scholar
  8. 8.
    E. C. Dawson, A. E. M. C. Dennison & B. K. VanWeemen, Steroids, 31, 357 (1978).CrossRefGoogle Scholar
  9. 9.
    C. Muro & G. Stabenfeldt, J. Endocrinology, 101, 41 (1984).CrossRefGoogle Scholar
  10. 10.
    D. A. Christensen & D. J. Kesler, Anim. Reprod. Sci., 7, 531 (1984).CrossRefGoogle Scholar
  11. 11.
    G. H. Parsons, Jr., “Methods in Enzymology,” Vol. 73, Academic Press, New York, 1981.Google Scholar
  12. 12.
    K. E. Hechemy & R. L. Anacker, J. Immunoassay, 4, 147 (1983).CrossRefGoogle Scholar
  13. 13.
    M. Suter, J. Immunol. Methods, 53, 103 (1982).CrossRefGoogle Scholar
  14. 14.
    J. Dobbins Place & H. R. Schroeder, J. Immunol. Methods, 48, 251 (1982).CrossRefGoogle Scholar
  15. 15.
    E. E. Howell, J. Nasser & K. J. Schray, J. Immunoassay, 2, 205 (1980).Google Scholar
  16. 16.
    E. Ishikawa, Y. Hamaguchi & M. Imegawa, J. Immunoassay, 1, 385 (1980).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • D. J. Kesler
    • 1
  • H. Khazali
    • 1
  • R. J. Favero
    • 1
  1. 1.Physiology Research Laboratory, Reproductive Engineering and Biotechnology, Department of Animal SciencesUniversity of IllinoisUrbanaUSA

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