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Electrophysiological Study of Recovery of Peripheral Nerves Regenerated by a Collagen-Glycosaminoglycan Copolymer Matrix

  • Albert Chang
  • Ioannis V. Yannas
  • Susanne Perutz
  • Howard Loree
  • Rajesh R. Sethi
  • Christian Krarup
  • Thorkild V. Norregaard
  • Nicholas T. Zervas
  • J. Silver

Abstract

We have studied the effects of variations in the structure of a collagen-glycosaminoglycan (CG) copolymer matrix on the regeneration of transected rat sciatic nerves. Silicone tubes ensheathing 10-mm lengths of CG copolymer were grafted between the transected sciatic nerve stumps. Empty and saline-filled silicone tubes, as well as autografts, were studied as controls. The mean pore diameter and the degradation rate of the copolymer in collagenase were independently varied to investigate how each affects regeneration. Electrophysiological properties of the regenerating motor nerve fibers innervating the plantar flexor muscles, were serially monitored over about 40 weeks following surgery. Rapidly degrading CG copolymers with pore channels oriented predominantly along the axes of the tubes induced regeneration with a success rate of 100% (n = 35). Although CG copolymers with axially-oriented pore channels that degraded slowly had a success rate as high as 96% (n = 23), the long-term electrophysiological results were markedly inferior to those obtained with the rapidly degrading grafts. In another study of axially-oriented pore structures, the level of recovery of long-term electrophysiological results was observed to increase monotonously as preliminary results showed that CG copolymers with pore channels predominantly oriented along the radial direction of the tubes had a success rate of only 50% (n = 6). Control groups of empty and saline-filled tubes had an aggregated success rate of 29% (n = 21). The ongoing study has shown that systematic physiochemical manipulation of simple chemical analogs of the extracellular matrix can be used to define substrate features which encourage regeneration.

Keywords

Sciatic Nerve Motor Unit Conduction Velocity Pore Channel Silicone Tube 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Albert Chang
    • 1
  • Ioannis V. Yannas
    • 1
  • Susanne Perutz
    • 1
  • Howard Loree
    • 1
  • Rajesh R. Sethi
    • 2
  • Christian Krarup
    • 2
  • Thorkild V. Norregaard
    • 3
  • Nicholas T. Zervas
    • 4
  • J. Silver
    • 5
  1. 1.Massachusetts Institute of TechnologyCambridgeUSA
  2. 2.Brigham and Women’s HospitalBostonUSA
  3. 3.Deaconess HospitalBostonUSA
  4. 4.Massachusetts General HospitalBostonUSA
  5. 5.Case Western Reserve UniversityClevelandUSA

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