Techniques for Measuring Carbonic Anhydrase Activity in Vitro

The Electrometric Delta pH and pH Stat Methods
  • Raymond P. Henry


The isozymes of carbonic anhydrase (CA) (EC catalyze the reversible hydration—dehydration of carbon dioxide and water, with H+ ions being transferred between the active site of the enzyme and a surrounding buffer.3,16,17 This results in a change in pH as the reaction proceeds toward equilibrium. With the advent of rapid-responding pH and reference electrodes coupled to sensitive pH meters, these changes in H+ ion concentration could be accurately measured. That measurement, which can be performed either directly or indirectly, forms the underlying principle upon which the electrometric methods for the assay of CA activity are built.


Carbonic Anhydrase Reaction Vessel Buffer Concentration Dehydration Reaction Carbonic Anhydrase Activity 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Raymond P. Henry
    • 1
  1. 1.Department of Zoology and Wildlife ScienceAuburn UniversityAuburnUSA

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