The Carbonic Anhydrases pp 153-159 | Cite as
Immunological Methods for Detection and Quantification of Carbonic Anhydrase Isozymes
Chapter
Abstract
The quality of the antibody is the most important factor in any immunological detection system, qualitative or quantitative. The ideal antibody has high affinity for its antigen and monospecificity. The response to a given protein varies greatly between animals, and proteins differ in immunogenicity. In our laboratory, we have found it easy to raise antisera against rat carbonic anhydrase (CA II) but much more difficult to produce high-affinity antibodies to rat CA III.
Keywords
Immunological Method Sodium Metabisulfite Excess Antibody Unlabeled Protein Carbonic Anhydrase Isozyme
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References
- 1.Bolton, A. E., and Hunter, W. M., 1973 J. Endocrino!. 55:xxx—xxxi.Google Scholar
- 2.Carter, N. D., Hewett-Emmett, D., Jeffery, S., and Tashian, R. E., 1981, FEBS Lett. 128: 114–118.PubMedCrossRefGoogle Scholar
- 3.Graham, R. C., and Karnovsky, M. J., 1966, J. Histochem. Cytochem. 14: 291–295.PubMedCrossRefGoogle Scholar
- 4.Laemli, Y. N., 1979, Nature (London) 227: 680–685.CrossRefGoogle Scholar
- 5.Leary, J. J., Brigati, D. J., and Ward, D. C., 1983, Proc. Natl. Acad. Sci. USA 80: 4045–4049.Google Scholar
- 6.Norgaard-Pedersen, B., 1973, Scand J. Immunol. 2 (Suppl. 1): 125–131.CrossRefGoogle Scholar
- 7.Shepherd, J. N., Spencer, N., Bidwell, D. E., and Voller, A., 1982, Clin. Biochem. 15: 248–253.PubMedCrossRefGoogle Scholar
- 8.Sraker, S. J., and Speck, J. C., 1978, Biochem. Biophys. Res. Commun. 80: 849–854.Google Scholar
- 9.Thorell, J. J., and Johansson, B. G., 1971, Biochim. Biophys. Acta 251: 363–369.Google Scholar
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© Springer Science+Business Media New York 1991