Immunological Methods for Detection and Quantification of Carbonic Anhydrase Isozymes
The quality of the antibody is the most important factor in any immunological detection system, qualitative or quantitative. The ideal antibody has high affinity for its antigen and monospecificity. The response to a given protein varies greatly between animals, and proteins differ in immunogenicity. In our laboratory, we have found it easy to raise antisera against rat carbonic anhydrase (CA II) but much more difficult to produce high-affinity antibodies to rat CA III.
KeywordsImmunological Method Sodium Metabisulfite Excess Antibody Unlabeled Protein Carbonic Anhydrase Isozyme
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