A Protein from Mammalian Cells that Recognizes Platinated DNA
The anticancer drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin), is believed to kill cells by blocking DNA replication or transcription (Roberts and Thomson, 1979; Sorenson and Eastman, 1988a, b). cis-DDP-DNA adducts have been shown to block DNA replication in vitro (Pinto and Lippard, 1985b; Heiger-Bernays et al., 1990) and in vivo (Harder and Rosenberg, 1970; Howie and Gale, 1970; Ciccarelli et al., 1985). Similar recent studies have shown that selected platinum adducts also inhibit RNA polymerases (Corda et al., 1991).
KeywordsHeLa Cell Platinum Compound Ammonium Sulfate Fraction Placental Extract Slow Migrate Band
Unable to display preview. Download preview PDF.
- Caradonna, J. P., and Lippard, S. J. 1984, Chemical and biological studies of cis-diamminedichloroplatinum(II) binding to DNA, in: “Platinum Coordination Compounds in Cancer Chemotherapy,” M. P. Hacker, E. B. Douple, and I. H. Krakoff, eds., Martinus Nijhoff, Boston.Google Scholar
- Donahue, B. A., Augot, M., Bellon, S. F., Treiber, D. K., Toney, J. H., Lippard, S. J., and Essigmann, J. M. 1990, Characterization of a DNA damage-recognition protein from mammalian cells that binds specifically to intrastrand d(GpG) and d(ApG) DNA adducts of the anticancer drug cisplatin, Biochemistry 29:5872.PubMedCrossRefGoogle Scholar
- Plooy, A. C. M., van Dijk, M., Berends, F., and Lohman, P. H. M. 1985, Formation and repair of DNA interstrand cross-links in relation to cytotoxicity and unscheduled DNA synthesis induced in control and mutant human cells treated with cis-diamminedichloroplatinum(II). Cancer Res. 45:4178.PubMedGoogle Scholar
- Toney, J. H., Donahue, B. A., Kellett, P. J., Bruhn, S. L., Essigmann, J. M., and Lippard, S. J. 1989, Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II), Proc. Natl. Acad. Sci. USA 86:8328.PubMedCrossRefGoogle Scholar