A Protein from Mammalian Cells that Recognizes Platinated DNA

  • Brian A. Donahue
  • Steven F. Bellon
  • Stephen J. Lippard
  • John M. Essigmann


The anticancer drug cis-diamminedichloroplatinum(II) (cis-DDP or cisplatin), is believed to kill cells by blocking DNA replication or transcription (Roberts and Thomson, 1979; Sorenson and Eastman, 1988a, b). cis-DDP-DNA adducts have been shown to block DNA replication in vitro (Pinto and Lippard, 1985b; Heiger-Bernays et al., 1990) and in vivo (Harder and Rosenberg, 1970; Howie and Gale, 1970; Ciccarelli et al., 1985). Similar recent studies have shown that selected platinum adducts also inhibit RNA polymerases (Corda et al., 1991).


HeLa Cell Platinum Compound Ammonium Sulfate Fraction Placental Extract Slow Migrate Band 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Brian A. Donahue
    • 1
    • 2
  • Steven F. Bellon
    • 1
  • Stephen J. Lippard
    • 1
  • John M. Essigmann
    • 1
    • 2
  1. 1.Department of ChemistryMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Whitaker College of Health Sciences and TechnologyMassachusetts Institute of TechnologyCambridgeUSA

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