Regulation of Cyclooxygenase Gene Expression in Vascular Endothelial Cells

  • Timothy Hla
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Cyclooxygenase (Cox) (E.C. 1.14.99.1) is a rate-limiting enzyme in the biosynthesis of prostaglandins and thromboxanes, collectively known as prostanoids (PG) (1). PG synthesis may be regulated by irreversible self-inactivation of Cox, whereby the enzyme only forms a limited amount of product, estimated to be 1500 moles product/mole enzyme (2). The self-inactivated Cox is rapidly degraded by proteases (3). Thus, the half-life of Cox is short, estimated to be in the order of a few minutes in vitro as well as in vivo Furthermore, cyclooxygenase enzyme levels are modulated by pro-and anti-inflammatory mediators. Many growth factors and cytokines induce PG synthesis by inducing the de novo synthesis of Cox protein (4,5,6,7). In contrast, the anti-inflammatory glucocorticoids such as dexamethasone (dex) inhibit the synthesis of Cox activity (8) and protein synthesis (9).

Keywords

Human Umbilical Vein Endothelial Cell Amino Acid Sequence Identity Chicken Embryo Fibroblast Indispensible Amino Acid Diploid Human Cell Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Timothy Hla
    • 1
  1. 1.Laboratory of Molecular BiologyJerome H. Holland for the Biomedical SciencesRockvilleUSA

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