Advertisement

Lipoxin Generation in Human Whole Blood: Monitoring by Electron-Capture NICI GC/MS

  • Charles N. Serhan
  • Damian A. Brezinski
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Mammalian lipoxygenase-derived products of arachidonic acid were first identified with cells of hematopoietic origins (reviewed in ref. 1). Products of these enzymatic pathways (e.g. leukotrienes) play critical roles in a wide range of physiologic responses (2). The lipoxins (LX) are an addition to the established family of bioactive eicosanoids. Members of the LX series contain a conjugated tetraene structure, can be generated by the interactions of individual lipoxygenases or by cell-cell interactions, and display a profile of bioactions unique among eicosanoids (reviewed in 2,3). Results from several laboratories now show that lipoxin A4 can block the proinflammatory actions of leukotriene B4 and the hemodynamic effects of leukotriene D4. LXA4 may also play an intracellular role in its cell of origin, since it activates isolated protein kinase C and has been found to inhibit IP3 formation (recently reviewed in ref. 3). Thus, as is the case with other bioactive eicosanoids, the availability of appropriate quantitative methods will play a critical role in assessing the temporal association between lipoxin formation and their potential involvement in both physiologic and pathophysiologic events.

Keywords

Methyl Formate Electron Impact Mass Spectrum Bioactive Eicosanoid Intracoronary Blood Dihydroxyeicosatetraenoic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    S. Yamamoto, Prostaglandins. Leukotrienes and Essential Fatty Acids: Reviews, 35, 219 (1989).CrossRefGoogle Scholar
  2. 2.
    B. Samuelsson, S.-E. Dahlén, J.A. Lindgren, C.A. Rouzer and C.N. Serhan, Science, 237, 1171 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    S.-E. Dahlén and C.N. Serhan, in: “Lipoxygenases and Their Products,” S. Crooke and A. Wong, eds., Academic, Orlando (1991).Google Scholar
  4. 4.
    R.J. Strife and R.C. Murphy, J. Chromatog, 305, 3 (1984).Google Scholar
  5. 5.
    K.A. Waddell, S.E. Barrow, C. Robinson, M.A. Orchard, C.T. Dollery and I.A. Blair, Biomed. Mass Spectrom, 11, 68 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    S.E. Barrow and G.W. Taylor, in: “Prostaglandins and Related Substances: A Practical Approach,” C. Benedetto, R.G. McDonald-Gibson, S. Nigam and T.F. Slater, eds., p. 99, IRL Press, Oxford (1987).Google Scholar
  7. 7.
    C.R. Pace-Asciak and S. Micallef, J. Chromatog, 310, 233 (1984).Google Scholar
  8. 8.
    E. Malle, J. Nimpf, H.J. Leis, H. Wurm, H. Gleispach and G.M. Kostner, Prostaglandins Leukotrienes and Medicine, 27, 53 (1987).CrossRefGoogle Scholar
  9. 9.
    I.A. Blair, A.R. Brash, J. Daugherty and G.A. Fitz-Gerald, Advances in Prostaglandin, Thromboxane and Leukotriene Research, 15, 61 (1985).Google Scholar
  10. 10.
    D.A. Brezinski and C.N. Serhan, Biol. Mass Spectrum, 20, 45 (1991).CrossRefGoogle Scholar
  11. 11.
    D.A. Brezinski, R.W. Nesto and C.N. Serhan, submitted for publication.Google Scholar
  12. 12.
    B.E. Marron, R.A. Spanevello, M.E. Elisseou, C.N. Serhan and K.C. Nicolaou, J. Org. Chem, 54, 5522 (1989).CrossRefGoogle Scholar
  13. 13.
    T. Herrmann, D. Steinhilber, J. Knospe and H.J. Roth, J. Chromatog, 428, 237 (1988).Google Scholar
  14. 14.
    P. Gresele, J. Arnout, M.C. Coene, H. Deckmyn and J. Vermylen, Biochem. Biophys. Res. Commun, 137, 334 (1986).PubMedCrossRefGoogle Scholar
  15. 15.
    F.J. Sweeney, J.D. Eskra, M.J. Ernest and T.J. Carty, Agents and Actions, 21, 393 (1987).PubMedCrossRefGoogle Scholar
  16. 16.
    C. Edenius, J. Haeggström and J.A. Lindgren, Biochem. Biophys. Res. Commun. 157, 801 (1988).PubMedCrossRefGoogle Scholar
  17. 17.
    C.N. Serhan and K.-A. Sheppard, J. Clin. Invest. 85, 772 (1990).PubMedCrossRefGoogle Scholar
  18. 18.
    S. Fiore and C.N. Serhan, J. Exp. Med. 172, 993 (1990).CrossRefGoogle Scholar
  19. 19.
    P. Borgeat, A. Odeimat and P. E. Poubelle, 7th International Conference on Prostaglandins and Related Compounds (abstract), p. 103 (1990).Google Scholar
  20. 20.
    A. Fradin, J.A. Zirrolli, J. Maclouf, L. Vausbinder, P.M. Henson and R.C. Murphy, J. Immunol., 143, 3680 (1989).PubMedGoogle Scholar
  21. 21.
    G.W. Vetrovec, Am. J. Cardiol. 64, 27E (1989).Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Charles N. Serhan
    • 1
  • Damian A. Brezinski
    • 1
  1. 1.Hematology Division, Department of MedicineBrigham and Women’s Hospital and Harvard Medical SchoolBostonUSA

Personalised recommendations