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Catalytic Properties and Reaction Mechanism of 5-Lipoxygenase

  • D. Riendeau
  • D. Denis
  • J.-P. Falgueyret
  • M. D. Percival
  • M. J. Gresser
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The 5-lipoxygenase from leukocytes catalyzes the oxidation of arachidonic acid to 5hydroperoxyeicosatetraenoic acid (5-HPETE) and leukotriene A4 (LTA4) as the first two steps of the leukotriene biosynthesis pathway. The reaction catalyzed by 5-lipoxygenase is similar to that of other mammalian and plant lipoxygenases, showing activation by the hydroperoxide product, kinetic lag phases and turnover-dependent inactivation, but with additional requirements for ATP, Ca2+1 and for a leukocyte protein (FLAP) presumably involved in the translocation of the enzyme to the membrane during cellular leukotriene production2.

Keywords

Lipid Hydroperoxide Soybean Lipoxygenase Acetohydroxamic Acid Hydroperoxide Product Propyl Methyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • D. Riendeau
    • 1
  • D. Denis
    • 1
  • J.-P. Falgueyret
    • 1
  • M. D. Percival
    • 1
  • M. J. Gresser
    • 1
  1. 1.Merck Frosst Centre for Therapeutic ResearchPointe Claire — DorvalCanada

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