Modulation of Cytokine Gene Expression by LTB4 and PAF: Transcriptional and Post-Transcriptional Regulation
In recent years, the potential for lipid mediators of inflammation, such as LTB4 and PAF, to modulate the production of cytokines has been demonstrated in several laboratories1–12. Lipid mediators and cytokines appear to play a role in various stages of inflammatory and immune responses, and may interact with each other to generate positive or negative feedback loops. Recent studies in our laboratories were aimed at elucidating the mechanisms through which LTB4 and PAF could modulate the production of interleukin (IL)1α, IL1β, IL6 and tumor necrosis factor α (TNF) by human monocytes and myeloid cell lines. Both transcriptional and post-transcriptional regulation of cytokine gene expression could be demonstrated.
KeywordsHuman Monocyte Lipid Mediator mRNA Accumulation Cytokine Gene Expression Tumor Necrosis Factor Production
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- 6.M. Rola-Pleszczynski. Immune modulation by PAF: effects on lymphocyte and monocyte functions, in: “New Trends in Lipid Mediator Research”, Braquet P., ed., Karger, Basel, (1988).Google Scholar
- 7.S.G. Ward, G.P. Lewis, J. Westwick. Platelet-activating factor stimulates interleukin-1 production by human adherent monocytes-macrophages, in: “New Trends in Lipid Mediator Research”, Braquet P, ed., Karger, Basel, (1988).Google Scholar
- 10.B. Pignol, S. Hénane, B. Sorlin, M. Rola-Pleszczynski, J.-M. MenciaHuerta, P. Braquet. Effect of long-term treatment with platelet-activating factor on IL 1 and IL 2 production by rat spleen cells. T. Immunol. 145: 980 (1990).Google Scholar
- 12.P. Poubelle, D. Gingras, C. Demers, C. Dubois, D. Harbour, J. Grassi and M. Rola-Pleszczynski. Platelet-activating factor (PAF-acether) enhances the concomitant production of tumour necrosis factor-alpha and interleukin 1 by subsets of human monocytes. Immunol. 72: 181, (1991).Google Scholar
- 15.M. Rola-Pleszczynski and J. Stankova. Leukotriene B4 enhances IL 6 production and IL 6 mRNA accumulation in human monocytes. Transcriptional and post-transcriptional mechanisms. Submitted J. Immunol. (1991).Google Scholar
- 16.M.E. Greenberg and T.P. Bender. Identification of newly transcribed RNA. In “Protocols in Molecular Biology, vol. 1”, F.M. Ausubel, R. Brent, R.F. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl eds. Wiley & Sons, N.Y. (1990).Google Scholar
- 17.P. Poubelle, J. Stankova, J. Grassi, M. Rola-Pleszczynski. Leukotriene B4 up-regulates IL-6 rather than IL-1 synthesis in human monocytes. In press, Agents and Actions (1991).Google Scholar
- 18.M. Rola-Pleszczynski. Immunoregulation by leukotrienes and Platelet-activating factor. in: “The Neuroendocrine-immune network.” Serem Freier, ed., CRC Press, (1990).Google Scholar
- 20.M. Rola-Pleszczynski, S. Turcotte, D. Gingras. Enhanced TNF production and cytotoxic activity of human monocytes primed with PAF: post-receptor events. in: “Molecular and cellular biology of cytokines”, Oppenheim, Powanda, Kluger, Dinnarello, eds., Alan R. Liss Inc., (1990).Google Scholar
- 22.C. Lacasse and M. Rola-Plesczcynski Immune regulation by PAF II. Mediation of suppression by cytokine-stimulated endothelial cells in vitro. J. Leuk, Biol. 49: 245 (1991).Google Scholar
- 23.M. Rola-Pleszczynski. Priming of human monocytes with PAF augments their production of tumor necrosis factor. J. Lipid Mediators 2: S77 (1990).Google Scholar
- 25.M. Rola-Pleszczynski, C. Pouliot, S. Turcotte, B. Pignol, P. Braquet, and L. Bouvrette. Immune regulation by platelet-activating factor. I. Induction of suppressor cell activity in human monocytes and CD8+ T cells and of helper cell activity in CD4+ T cells. J. Immunol. 140: 3547 (1988).PubMedGoogle Scholar