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Influence of PGE2 and LTB4 on CD8+ Thymocytes

  • Norbert Gualde
  • Richard Daculsi
  • Isabelle Hostein
  • Dominique Vaillier
  • Frédéric Buffière
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Arachidonic acid oxygenation products were considered as mediators mostly involved in inflammation, hypersensitivity and pain. Recently eicosanoids have been shown to participate in the immunoregulation. At the very beginning, investigators were interested by studying the role of arachidonic acid derivatives on differentiated lymphocytes obtained from the blood or variable peripheral lymphoid organs, more recently some studies focused on the effect of these metabolites on differentiation and maturation of immature lymphoid cells such as thymocytes. Since the thymus plays a central role for T cell physiology and function [1,2] the question remains to know if arachidonic acid metabolites which are producted within the thymus have a critical function in determining the differentiation and maturation of thymocytes. For instance it was reported that eicosanoids are produced by thymic macrophages [3,4], phagocytic cells of the thymic reticulum [5], thymic epithelial cells [6] and thymic nurse cells [7]. The production of eicosanoids by thymocytes themselves is more controversial [8, 9, 10]. Therefore we were interested in the study of eicosanoids production by the cells of the thymus microenvironment trying to determine what is the actual production of arachidonic acid derivatives whithin the thymus. For that purpose we produced thymus-macrophage hybridoma and assessed the synthesis of eicosanoids, and because the production of lipoxygenase metabolites is less clear we also studied the 5-lipoxygenase mRNA expression among thymic cells.

Keywords

Thymic Epithelial Cell Arachidonic Acid Metabolite Thymidine Uptake Immature Thymocyte Thymus Microenvironment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Norbert Gualde
    • 1
    • 2
  • Richard Daculsi
    • 1
  • Isabelle Hostein
    • 1
  • Dominique Vaillier
    • 1
  • Frédéric Buffière
    • 1
    • 3
  1. 1.URA CNRS 1456Université de Bordeaux 2BordeauxFrance
  2. 2.Fondation BergoniéBordeauxFrance
  3. 3.Centre Régional de Transfusion SanguineBordeauxFrance

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