Metabolism of Arachidonic Acid by Purified Lung Epithelial Cells

  • Pierre Sirois
  • Stéphane Prié
  • Johanne Laporte
  • Karim Maghni
  • Pierre Borgeat
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


The airway epithelium is composed of a number of cell types with highly selective functions. The mucus cells, the Clara cells and, to a certain extent, the type II pneumocytes have major secretory roles. Whereas mucus cells are responsible for the secretion of the mucoproteins covering large airways (St. George et al., 1988), type II pneumocytes (Bantenburg and Val Go1de, 1979; King, 1982) and Clara cells (Niden, 1967) secrete surfactant which cover small airways and maintain surface tension. The ciliated cells allow mucociliary transport (Gail and Lenfant, 1983) and the basal cells are involved in the attachment of columnar cells to the lamina propria (Evans and Plopper, 1988). Clara cells are the primary sites of pulmonary defense mechanism against toxicants and are involved in the activation and detoxification of xenobiotics by their active P-450 cytochrome monooxygenase pathway (Boyd, 1977). In addition, epithelial cells from various species including man, rabbit and rat were shown to release arachidonic acid metabolites which modulate mucus secretion (Gail and Lenfant, 1983) and airway caliber (Sirois et al., 1987). The purpose of the following investigation was to study the metabolism of arachidonic acid by purified populations of guinea pig epithelial cells.


Arachidonic Acid Airway Epithelial Cell Ciliated Cell Mucus Cell Clara Cell 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Pierre Sirois
    • 1
  • Stéphane Prié
    • 1
  • Johanne Laporte
    • 1
  • Karim Maghni
    • 1
  • Pierre Borgeat
    • 2
  1. 1.Department of Pharmacology Faculty of MedicineUniversity of SherbrookeSherbrooke(P.Q.)Canada
  2. 2.Inflammation et Immunologie-Rhumatologie, CHULQuebecCanada

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