Eicosanoid/Signal Transduction Interactions: Evidence of Two Induced Pathways of Phosphoinositide Biosynthesis by LTB4 in Cultured Human Epidermal Cells
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)
Hydrolysis of phosphatidylinositol 4,5-biphosphate (PtdIns4,5P2) resulting in the generation of two “putative second messengers”: inositol 1,4,5-triphosphate (Ins1,4,5P3) and diacylglycerol (DAG) is now recognized as an essential pathway for generating modulators of a wide variety of hormonal signals (Hokin, 1985; Berridge, 1984; Nishizuka, 1984). The replenishment of cellular PtdIns4,5P2 depends on the sequential phosphorylation of the 4- and 5-hydroxy groups of the inositol ring by PtdIns and PtdInsP kinases respectively, implying that the mode of phosphorylation of the phosphoinositides is an important aspect in the generation of molecules involved in the signal transduction systems. An outline of the inositol phospholipid cascade showing the conventional pathway as well as the putative 3-kinase pathway resulting in phosphatidylinositol 3,4,5-trisphosphate (PtdIns3,4,5P3) is shown in Fig. 1.
KeywordsInositol Phosphate Inositol Trisphosphate Radioactive Peak Inositol Phospholipid Potassium Oxalate
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- Bradford P.G., and Rubin R.P., 1985, Leukotriene B4 stimulates phospholipase C-mediated formation of inositol trisphosphate in neutrophils: Implications for convergence of the phospholipase A2 and C pathways, Adv. Prostag. Throm. Leuko. Res. 15: 105–108.Google Scholar
- Mong S., Hoffman K., Wu H-L. and Crooke S, 1987, Leukotriene-induced hydrolysis of inositol phospholipids in guinea pig lung: Mechanism of signal transduction for leukotrine-D4 receptors, Mol. Pharm. 31: 35–41.Google Scholar
- Traynor-Kaplan A.E., Thompson B.L., Harris A.L., Taylor P., Omann G.M. and SklarGoogle Scholar
- L.A., 1989, Transient increase in phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol trisphosphate during activation of human neutrophils, J. Biol. Chem. 264: 15668–15673.Google Scholar
- Whitman M, Kaplan D., Roberts T. and Cantley L., 1987, Evidence for two distinct phosphatidylinositol kinases in fibroblasts: Implications for cellular regulation. Biochem. J. 247: 165–174.Google Scholar
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