Lipoxygenase, Cyclooxygenase and Leukotriene A4 Hydrolase: Quantitative Polymerase Chain Reaction and Expression Studies
Arachidonic acid is converted to an array of potent biological mediators such as prostaglandins, leukotrienes and thromboxane in a tissue and cëll specific fashion (1,2). The enzymes that catalyze these transformations, leukocyte 5-lipoxygenase, reticulocyte/eosinophil 15-lipoxygenase, platelet 12-lipoxygenase, prostaglandin G/H synthase (cyclooxygenase) and leukotriene A4 hydrolase have been characterized and their sequences have been deduced by cloning of their respective complementary DNAs (3–10). Besides substrate availability, it has become increasingly evident that transcriptional and translational control as well as “suicide-type” inactivation are critical factors that regulate eicosanoid generation (11). We have now established a quantitative polymerase chain reaction (PCR) assay as a means to assess this regulation at the mRNA level.
KeywordsPolymerase Chain Reaction Assay Quantitative Polymerase Chain Reaction Assay Vesicular Gland Platelet Cyclooxygenase Method Quantitative Polymerase Chain Reaction
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- 5.Sigal, E., Craik, C.S., Highland, E., Grunberger, D., Costello, L.L., Dixon, R.A.F., and Nadel, J.A. (1988) Biochem. Biophys. Res. Commun 157, 457464.Google Scholar
- 10.Minami, M., Ohno, S., Kawasaki, H., Radmark, O., Samuelsson, B., Jornvall, H., Shimizu, T., Seyama, Y., and Suzuki, K. (1987) J. Biol. Chem. 262, 1387313876.Google Scholar
- 12.Funk, C.D., and FitzGerald, G.A. (1991) J. Biol. Chem. 266 in press.Google Scholar