Lipoxygenase, Cyclooxygenase and Leukotriene A4 Hydrolase: Quantitative Polymerase Chain Reaction and Expression Studies

  • Colin D. Funk
  • Xin-Sheng Chen
  • Garret A. Fitzgerald
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Arachidonic acid is converted to an array of potent biological mediators such as prostaglandins, leukotrienes and thromboxane in a tissue and cëll specific fashion (1,2). The enzymes that catalyze these transformations, leukocyte 5-lipoxygenase, reticulocyte/eosinophil 15-lipoxygenase, platelet 12-lipoxygenase, prostaglandin G/H synthase (cyclooxygenase) and leukotriene A4 hydrolase have been characterized and their sequences have been deduced by cloning of their respective complementary DNAs (3–10). Besides substrate availability, it has become increasingly evident that transcriptional and translational control as well as “suicide-type” inactivation are critical factors that regulate eicosanoid generation (11). We have now established a quantitative polymerase chain reaction (PCR) assay as a means to assess this regulation at the mRNA level.

Keywords

Polymerase Chain Reaction Assay Quantitative Polymerase Chain Reaction Assay Vesicular Gland Platelet Cyclooxygenase Method Quantitative Polymerase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Samuelsson, B. (1983) Science 220, 568–575.PubMedCrossRefGoogle Scholar
  2. 2.
    Samuelsson, B. and Funk, C.D. (1989) J. Biol. Chem. 264, 19469–19472.PubMedGoogle Scholar
  3. 3.
    Matsumoto, T., Funk, C.D., Radmark, O., Hoog, J.-O., Jomvall, H., and Samuelsson, B. (1988) Proc. Natl. Acad. Sci. USA 85, 26–30; Correction (1988) Proc. Natl. Acad. Sci. USA 85, 3406.CrossRefGoogle Scholar
  4. 4.
    Dixon, R.A.F., Jones, R.E., Diehl, R.E., Bennett, C.D., Kargman, S., and Rouzer, C.A. (1988) Proc. Natl. Acad. Sci. USA 85, 416–420.CrossRefGoogle Scholar
  5. 5.
    Sigal, E., Craik, C.S., Highland, E., Grunberger, D., Costello, L.L., Dixon, R.A.F., and Nadel, J.A. (1988) Biochem. Biophys. Res. Commun 157, 457464.Google Scholar
  6. 6.
    Funk, C.D., Furci, L., and FitzGerald, G.A. (1990) Proc. Natl. Acad. Sci. USA 87 5638–5642.CrossRefGoogle Scholar
  7. 7.
    DeWitt, D.L., and Smith, W.L. (1988) Proc. Natl. Acad. Sci. USA 85, 1412–1416.CrossRefGoogle Scholar
  8. 8.
    Merlie, J.P., Fagan, D., Mudd, J., and Needleman, P. (1988) J. Biol Chem. 263, 3550–3553.PubMedGoogle Scholar
  9. 9.
    Funk, C.D., Radmark, O., Fu, J.Y., Matsumoto, T., Jornvall, H., Shimizu, T., and Samuelsson, B. (1987) Proc. Natl. Acad. Sci. USA 84 6677–6681.CrossRefGoogle Scholar
  10. 10.
    Minami, M., Ohno, S., Kawasaki, H., Radmark, O., Samuelsson, B., Jornvall, H., Shimizu, T., Seyama, Y., and Suzuki, K. (1987) J. Biol. Chem. 262, 1387313876.Google Scholar
  11. 11.
    Fitzpatrick, F.A. (1991) Trends Cardiovascular Med. 1 81–86.CrossRefGoogle Scholar
  12. 12.
    Funk, C.D., and FitzGerald, G.A. (1991) J. Biol. Chem. 266 in press.Google Scholar
  13. 13.
    Funk, C.D., Funk, L.B., Kennedy, M.E., Pong, A.S. and FitzGerald, G.A. (1991) FASEB J. 5, 2304–2312.PubMedGoogle Scholar
  14. 14.
    Smith, D.B., and Johnson, K.S. (1988) Gene 87, 31–40.CrossRefGoogle Scholar
  15. 15.
    Yokoyama, C., and Tanabe, T. (1989) Biochem. Biophys. Res. Commun. 165, 888–894.CrossRefGoogle Scholar
  16. 16.
    DeWitt, D.L., El-Harith, E.A., Kraemer, S.A., Andrews, M.J., Yao, E.F., Armstrong, R.L., and Smith, W.L. (1990) J. Biol. Chem. 265, 5192–5198.PubMedGoogle Scholar
  17. 17.
    Xie, W., Chipman, J.G., Robertson, D.L., Erikson, R.L. and Simmons, D.L. (1991) Proc. Natl. Acad. Sci. USA 88, 2692–2696.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Colin D. Funk
    • 1
  • Xin-Sheng Chen
    • 1
  • Garret A. Fitzgerald
    • 1
  1. 1.Division of Clinical PharmacologyVanderbilt UniversityNashvilleUSA

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