Abstract
Arachidonic acid is converted to an array of potent biological mediators such as prostaglandins, leukotrienes and thromboxane in a tissue and cëll specific fashion (1,2). The enzymes that catalyze these transformations, leukocyte 5-lipoxygenase, reticulocyte/eosinophil 15-lipoxygenase, platelet 12-lipoxygenase, prostaglandin G/H synthase (cyclooxygenase) and leukotriene A4 hydrolase have been characterized and their sequences have been deduced by cloning of their respective complementary DNAs (3–10). Besides substrate availability, it has become increasingly evident that transcriptional and translational control as well as “suicide-type” inactivation are critical factors that regulate eicosanoid generation (11). We have now established a quantitative polymerase chain reaction (PCR) assay as a means to assess this regulation at the mRNA level.
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Funk, C.D., Chen, XS., Fitzgerald, G.A. (1991). Lipoxygenase, Cyclooxygenase and Leukotriene A4 Hydrolase: Quantitative Polymerase Chain Reaction and Expression Studies. In: Bailey, J.M. (eds) Prostaglandins, Leukotrienes, Lipoxins, and PAF. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0727-1_11
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DOI: https://doi.org/10.1007/978-1-4899-0727-1_11
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