Robotic Thin Layer Chromatography Instrument for Synthetic Chemistry
We have constructed a second generation instrument for performing automated thin layer chromatography (TLC). The TLC instrument consists of four dedicated stations for 1) plate dispensing, 2) sample application, 3) plate development, and 4) densitometry. A robot is used to move TLC plates among stations. The TLC instrument functions either as a stand-alone unit or as one analytical module in a robotic workstation for synthetic chemistry. An integrated hardware and software architecture enables automatic TLC analysis of samples produced concurrently from synthetic reactions in progress on the workstation. The combination of fixed automation and robotics gives a throughput of 12 TLC samples per hour. From these results a blueprint has emerged for an advanced automated TLC instrument with far greater throughput and analytical capabilities.
KeywordsHigh Pressure Liquid Chromatography Thin Layer Chromatography Plate Generation Instrument Sample Application Sample Storage
Unable to display preview. Download preview PDF.
- 2.L. A. Corkan, J. S. Lindsey, Design Concepts for Synthetic Chemistry Workstations, in Advances in Laboratory Automation Robotics,J. R. Strimaitis and G. L. Hawk, Editors; Zymark Corp., Hopkinton, Mass., 1990, Vol. 6,pp. 477.Google Scholar
- b) T. H. Maugh, TLC: The Overlooked Alternative, Science, 1982, 216, 161.Google Scholar
- 4.For an example of partial robotic automation of TLC, see: N. Morokoshi, S. Inada, S. Koda, Y. Morimoto, Automation of Thin Layer Chromatography by use of Robotics, in Advances in Laboratory Automation Robotics,J. R. Strimaitis and G. L. Hawk, Editors; Zymark Corp., Hopkinton, Mass., 1988, Vol. 4,pp. 95.Google Scholar
- 5.Camag Scientific Inc., Wrightsville, NC.Google Scholar
- 6.For general reviews of quantitative TLC, see: a) C. F. Poole, S. Khatib, Quantitative Thin-Layer Chromatography, in Quantitative Analysis using Chromatographic Techniques, E. Katz ( Wiley, New York ) 1987; 193.Google Scholar
- b) C. F. Poole, S. Khatib, T. A. Dean, High Performance Thin-Layer Chromatography, Chromatogr. Forum May-June 1986, 1, 27.Google Scholar