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Methods of Isolating Trophozoites and Cysts

  • Dennis E. Feely

Abstract

The culturing techniques discussed in Chapter 6 have facilitated the study of Giardia duodenalis trophozoites. However, only G. duodenalis from relatively few host species have been cultured. G. muris, G. agilis, and G. duodenalis from other hosts have not been cultured successfully. The study of the trophozoites of these species requires their isolation directly from the host. Mucosal smears, intestinal washings, and fecal smears are suitable for some morphological studies but are highly contaminated with debris.

Keywords

Sucrose Density Gradient Watch Glass Iodoacetic Acid Prairie Vole Sucrose Density Gradient Centrifugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Dennis E. Feely
    • 1
  1. 1.Department of Oral Biology and Basic Sciences, College of DentistryUniversity of Nebraska Medical CenterLincolnUSA

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