Metabolism of Trophozoites

  • Donald G. Lindmark
  • Edward L. Jarroll

Abstract

The protozoon Giardia lamblia is a member of the order Diplomonadida which includes a variety of binucleate flagellate parasites typically inhabiting the intestinal tract of mammals, birds, and reptiles (Kulda and Nohynkova, 1978). Each organism has eight flagella and a striated disc which might play a role in its attachment to enterocytes (Holberton, 1974). Although it lacks mitochondria and microbodies, G. lamblia is a typical eukaryote which contains lysosomelike organelles that give a positive histochemical reaction for acid phosphatase and can accumulate ferritin (Bockman and Winborn, 1968). This cellular organization, characterized by the absence of mitochondria, is also found in other groups of parasitic protozoa, including the Entamoeba spp. and trichomonads (Lindmark and Müller, 1973).

Keywords

Malate Dehydrogenase Sterol Ester Entamoeba Histolytica Orotic Acid Fumarate Hydratase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bockman, D. E., and Winborn, W. W., 1968, Electron microscope localization of exogenous ferritin within vacuoles of Giardia lamblia, J. Protozool. 15:26–30.PubMedGoogle Scholar
  2. Friend, D. S., 1966, The fine structure of Giardia muris, J. Cell Biol. 29:317–331.PubMedCrossRefGoogle Scholar
  3. Hill, B., Kilsby, J., Rogerson, G. W., Mclntosh, R. T., and Ginger, C. D., 1981, The enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths, Mol. Biochem. Parasitol. 2:128–134.CrossRefGoogle Scholar
  4. Holberton, D. V., 1974, Attachment of Giardia—A hydrodynamic model based on flagellar activity, J. Exp. Biol. 60:207–221.PubMedGoogle Scholar
  5. Jarroll, E. L., Muller, P. J., Meyer, E. A., and Morse, S. A., 1981, Lipid and carbohydrate metabolism of Giardia lamblia, Mol Biochem. Parasitol. 2:187–196.PubMedCrossRefGoogle Scholar
  6. Kulda, J., and Nohynkova, E., 1978, Flagellates of the human intestine and of intestines of other species, in: Parasitic Protozoa, Vol. II (J.P. Kreier, ed.), pp. 2–139, Academic Press, New York.Google Scholar
  7. Lindmark, D. G., 1976, Acetate production by Tritrichomonas foetus, in: Biochemistry of Parasites and Host-Parasite Interrelationships (H. Van den Bossche, ed.), pp. 15–21, Elsevier/North-Holland, Amsterdam.Google Scholar
  8. Lindmark, D. G., 1980a, Energy metabolism of the anaerobic protozoon, Giardia lamblia, Mol. Biochem. Parasitol. 1:1–12.Google Scholar
  9. Lindmark, D. G., 1980b, Hydrolases of Giardia lamblia, American Society of Parasitologists, Berkeley.Google Scholar
  10. Lindmark, D. G., and Jarroll, E. L., 1982, Pyrimidine metabolism of Giardia lamblia, Mol. Biochem. Parasitol. 5:291–296.PubMedCrossRefGoogle Scholar
  11. Lindmark, D. G., and Müller, M., 1973, Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate, Tritrichomonas foetus, J. Biol. Chem. 248:7724–7728.Google Scholar
  12. Meyer, E. A., 1976, Giardia lamblia: Isolation and axenic cultivation, Exp. Parasitol. 39:101–105.PubMedCrossRefGoogle Scholar
  13. Montalvo, F. E., Reeves, R. E., and Warren, L. G., 1971, Aerobic and anaerobic metabolism in Entamoeba histolytica, Exp. Parasitol. 30:249–256.PubMedCrossRefGoogle Scholar
  14. Müller, M., 1973, Biochemical cytology of trichomonad flagellates. I. Subcellular localization of hydrolases, dehydrogenases and catalase in Tritrichomonas foetus, J. Cell Biol. 243:453–474.CrossRefGoogle Scholar
  15. Müller, M., 1976, Carbohydrate metabolism of trichomonads, in: Biochemistry of Parasites and Host-Parasite Interrelationships (H. Van de Bossche, ed.), pp. 1–14, Elsevier/North-Holland, Amsterdam.Google Scholar
  16. Reeves, R., 1972, Carbohydrate metabolism of Entamoeba histolytica, in: Comparative Biochemistry of Parasites (H. Van den Bossche, ed.), pp. 351–358, Academic Press, New York.CrossRefGoogle Scholar
  17. Reeves, R. E., Warren, L. G., Susskind, B., and Lo, H., 1977, An energy conserving pyruvate to acetate pathway in Entamoeba histolytica: Pyruvate synthase and a new acetate thiokinase, J. Biol. Chem. 252:726–731.PubMedGoogle Scholar
  18. Ryley, J. F., 1955, Studies on the metabolism of protozoa. V. Metabolism of the parasitic flagellate, Tritrichomonas foetus, Biochem. J. 59:361–369.PubMedGoogle Scholar
  19. Uyeda, K., and Rabinowitz, J. R., 1971, Pyruvate-ferredoxin oxidoreductase of Clostridium acidiurici, J. Biol. Chem. 246:9–20.Google Scholar
  20. Weinbach, E. C, Claggett, E. C, Keister, D. B. and Diamond, L. S., 1980, Respiratory metabolism of Giardia lamblia, J. Parasitol. 66:347–350.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Donald G. Lindmark
    • 1
  • Edward L. Jarroll
    • 1
  1. 1.Department of Preventive Medicine, New York State College of Veterinary MedicineCornell UniversityIthacaUSA

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