Detection of Giardia Cysts in Drinking Water

State of the Art
  • Walter Jakubowski


The detection, identification, and enumeration of microbiological contaminants, and in the present discussion specifically Giardia cysts in water supplies, are of concern to workers in a number of different disciplines. The microbiologist and the epidemiologist need to know the source of contamination, the level of contamination, the relationship to indicator organisms, and the reservoirs of infection. The engineer and the utility operator need to know how to remove Giardia or how to inactivate it by water treatment. Those responsible for administering drinking water programs want to know where and when the organism occurs in water, the suitability and availability of monitoring methods, and whether or not maximum contaminant levels, treatment requirements or guidance should be developed.


Sewage Sample Filter Holder Filter Cartridge Giardia Cyst Filter Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. APHA—AWWA—WPCF, 1981, Standard Methods for the Examination of Water and Wastewater, 15th ed., American Public Health Association, Washington, D.C.Google Scholar
  2. Barbour, A. G., Nichols, C. R., and Fukushima, T., 1976, An outbreak of giardiasis in a group of campers, Am. J. Trop. Med. Hyg. 25:384–389.PubMedGoogle Scholar
  3. Bingham, A. K., and Meyer, E. A., 1979, Giardia excystation can be induced in vitro in acidic solutions, Nature (London) 277:301–302.CrossRefGoogle Scholar
  4. Bingham, A. K., Jarroll, E. L., Jr., Meyer, E. A., and Radulescu, S., 1979, Giardia sp.: Physical factors of excystation in vitro, and excystation vs. eosin exclusion as determinants of viability, Exp. Parasitol. 47:284–291.PubMedCrossRefGoogle Scholar
  5. Black, R. E., Dykes, A. C, Sinclair, S. P., and Wells, J. G., 1977, Giardiasis in day-care centers: Evidence of person-to-person transmission, Pediatrics 60:486–491.PubMedGoogle Scholar
  6. Black, R. E., Dykes, A. C., Anderson, K. E., Wells, J. G., Sinclair, S. P., Gary, G. W., Jr., Hatch, M. H., and Gangarosa, E. J., 1981, Handwashing to prevent diarrhea in day-care centers, Am. J. Epidemiol. 113:445–451.PubMedGoogle Scholar
  7. Centers for Disease Control, 1977, Intestinal Parasite Surveillance Annual Summary, 1976.Google Scholar
  8. Chang, S. L., and Kabler, P. W., 1956, Detection of cysts of Endamoeba histolytica in tap water by the use of membrane filter, Am. J. Hyg. 64:170–180.PubMedGoogle Scholar
  9. Colorado Disease Bulletin, 1981, Do skiers catch giardiasis in Colorado?, Vol. IX(4).Google Scholar
  10. Craun, G. F., 1979, Waterborne giardiasis in the United States: A review, Am. J. Public Health 69:817–819.PubMedCrossRefGoogle Scholar
  11. Danciger, M., and Lopez, M., 1975, Numbers of Giardia in the feces of infected children, Am. J. Trop. Med. Hyg. 24:237–242.PubMedGoogle Scholar
  12. Dykes, A. C, Juranek, D. D., Lorenz, R. A., Sinclair, S., Jakubowski, W., and Davies, R., 1980, Municipal waterborne giardiasis: An epidemiologic investigation, Ann. Intern. Med. 92:165–170.PubMedCrossRefGoogle Scholar
  13. Frost, F., Plan, B., and Liechty, B., 1980, Giardia prevalence in commercially trapped mammals, J. Environ. Health 42:245–249.Google Scholar
  14. Healy, G. R., 1979, The presence and absence of Giardia lamblia in studies on parasite prevalence in the U.S.A., in: Waterborne Transmission of Giardiasis (W. Jakubowski and J. C. Hoff, eds.), Environmental Protection Agency 600/9-79-001 pp. 92-103.Google Scholar
  15. Jakubowski, W., and Ericksen, T. H., 1979, Methods for detection of Giardia cysts in water supplies, in: Waterborne Transmission of Giardiasis (W. Jakubowski and J. C. Hoff, eds.), Environmental Protection Agency 600/9-79-001 pp. 193-210.Google Scholar
  16. Jarroll, E. L., Jr., Bingham, A. K., and Meyer, E. A., 1981, Effect of chlorine on Giardia lamblia cyst viability, Appl. Environ. Microbiol. 41:483–487.PubMedGoogle Scholar
  17. Juranek, D. D., 1979, In discussion of: Water filtration techniques for removal of Giardia cysts and cyst models by G. S. Logsdon, J. M. Symons, and R. L. Hoye, Jr., in: Waterborne Transmission of Giardiasis (W. Jakubowski and J. C. Hoff, eds.), Environmental Protection Agency 600/9-79-001 pp. 240-256.Google Scholar
  18. Kirner, J. C, Littler, J. D., and Angelo, L. A., 1978, A waterborne outbreak of giardiasis in Camas, Washington, J. Am. Water Works Assoc. 70:35–40.Google Scholar
  19. Lippy, E. C, 1978, Tracing a giardiasis outbreak at Berlin, New Hampshire, J. Am. Water Works Assoc. 70:512–520.Google Scholar
  20. Logsdon, G. S., Symons, J. M., Hoye, R. L., Jr., and Arozarena, M. M., 1981, Alternative filtration methods for removal of Giardia cysts and cyst models, J. Am. Water Works Assoc. 73:111–118.Google Scholar
  21. Luchtel, D. L., Lawrence, W. P., and DeWalle, F. B., 1980, Electron microscopy of Giardia lamblia cysts, Appl. Environ. Microbiol. 40:821–832.PubMedGoogle Scholar
  22. Meyer, E. A., 1976, Giardia lamblia: Isolation and axenic cultivation, Exp. Parasitol. 30:101–105.CrossRefGoogle Scholar
  23. Moore, G. T., Cross, W. M., McGuire, D., Mollohan, C. S., Gleason, N. N., Healy, G. R., and Newton, L. H., 1969, Epidemic giardiasis at a ski resort, N. Engl. J. Med. 281:402–407.PubMedCrossRefGoogle Scholar
  24. Murray, C. R., and Reeves, E. B., 1977, Estimated use of water in the United States in 1975, Geological Survey Circular 765, U.S. Geological Survey, Department of Interior, Arlington, Va.Google Scholar
  25. Osterholm, M. T., Forfang, J. C, Ristinen, T. L., Dean, A. G., Washburn, J. W., Godes, J. R., Rude, R. A., and McCullough, J. G., 1981, An outbreak of foodborne giardiasis, N. Engl. J. Med. 304:24–28.PubMedCrossRefGoogle Scholar
  26. Rice, E. W., and Schaefer, F. W., 1981, An improved in vitro excystation procedure for Giardia lamblia cysts, J. Clin. Microbiol. 14(6):709–710.PubMedGoogle Scholar
  27. Shaw, P. K., Brodsky, R. E., Lyman, D. O., Wood, B. T., Hibler, C. P., Healy, G. R., MacLeod, K. I. E., Stahl, W., and Schultz, M. G., 1977, A communitywide outbreak of giardiasis with evidence of transmission by a municipal water supply, Ann. Intern. Med. 87:426–432.PubMedCrossRefGoogle Scholar
  28. Tsuchiya, H., 1931, A study on variabilities in dimensions and numbers of discharged cysts of Giardia lamblia (Stiles, 1915) from day to day under normal conditions, Am. J. Hyg. 13:544–567.Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Walter Jakubowski
    • 1
  1. 1.Health Effects Research LaboratoryUnited States Environmental Protection AgencyCincinnatiUSA

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