Human Immune Responses to Giardia lamblia

  • Phillip D. Smith

Abstract

Epidemiologic, clinical, and experimental observations indicate that man develops an immune response to Giardia lamblia. Studies of several giardiasis outbreaks (Moore et al., 1969; Barbour et al., 1976) show that individuals repeatedly exposed to G. lamblia have a lower incidence of infection and symptoms than newly exposed individuals, suggesting that prior exposure imparts partial resistance to reinfection. Clinical observations (Hughes et al., 1971; Ajdukiewicz et al., 1972; Ament et al., 1973; Brown et al., 1973; Hermans et al., 1976) of hypogammaglobulinemic patients reveal that these individuals have an increased prevalence of giardiasis, emphasizing the potential importance of antibody in the immunity to this parasite. In addition, a high prevalence of G. lamblia infection in homosexual men (Meyers et al., 1977; Hurwitz and Owen, 1978; Schmerin et al., 1978) suggests that cellular immunity may participate in man’s response to Giardia, since many homosexuals have an acquired T-cell defect (Masur et al., 1982; Siegal et al., 1982; Gottlieb et al., 1982). As discussed in Chapter 11, recent investigations (Roberts-Thomson et al., 1976; Stevens et al., 1978; Roberts-Thomson and Mitchell, 1978) in experimental G. muris infection in mice confirm the role of immunity in the murine response to Giardia, Recently, macrophages have also been implicated in immunity to this parasite, since macrophages of both mice (Owen et al., 1981) and rabbits (Radulescu and Meyer, 1981) are capable of phagocytosing Giardia.

Keywords

Human Immune Response Giardia Lamblia ADCC Assay Peripheral Blood Granulocyte Minor Antigen 
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References

  1. Ajdukiewicz, A. B., Youngs, G. R., and Bouchier, I. A. D., 1972, Nodular lymphoid hyperplasia with hypogammaglobulinaemia, Gut 13:589–595.PubMedCrossRefGoogle Scholar
  2. Ament, M. E., Ochs, H. D., and Davis, D. D., 1973, Structure and function of the gastrointestinal tract in primary immunodeficiency syndromes: A study of 39 patients, Medicine 52:248–277.CrossRefGoogle Scholar
  3. Barbour, A. G., Nichols, C. R., and Fukushima, T., 1976, An outbreak of giardiasis in a group of campers, Am. J. Trop. Med. Hyg. 25:384–389.PubMedGoogle Scholar
  4. Bellamy, J. E., and Nielson, N. O., 1974, Immune mediated emigration of neutrophils into the lumen of the small intestine, Infect. Immun. 9:615–619.PubMedGoogle Scholar
  5. Brandborg, L., Tankersley, C. B., Gottlieb, S., Barancik, M., and Sartor, V., 1967, Histological demonstration of mucosal invasion by Giardia lamblia in man, Gastroenterology 52:143–150.PubMedGoogle Scholar
  6. Brown, W. R., Butterfield, D., Savage, D., and Tada, T., 1973, Clinical, microbiological, and immunological studies in patients with immunoglobulin deficiencies and gastrointestinal disorders, Gut 13:441–449.CrossRefGoogle Scholar
  7. Dean, D. A., Wistar, R., and Murrell, K. D., 1974, Combined in vitro effects of rat antibody and neutrophilic leukocytes on Schistosoma mansoni, Am. J. Trop. Med. Hyg. 23:420–428.PubMedGoogle Scholar
  8. Diamond, L. S., Harlow, D. R., and Cunnick, C. C, 1978, A new medium for axenic cultivation of Entamoeba histolytica and other Entamoeba, Trans. R. Soc. Trop. Med. Hyg. 72:431–433.PubMedCrossRefGoogle Scholar
  9. Ellner, J. J., and Mahmoud, A. A. F., 1979, Killing of schistosomula of Schistosoma mansoni by normal human monocytes, J. Immunol. 123:949–951.PubMedGoogle Scholar
  10. Gottlieb, M. S., Schroff, R., Schanker, H. M., Weisman, J. D., Fan, P. T., Wolf, R. A., and Saxon, A., 1982, Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: Evidence of a new acquired cellular immunodeficiency, N. Engl. J. Med. 305:1425–1431.CrossRefGoogle Scholar
  11. Heatley, R. V., and Bienenstock, J., 1982, Luminal lymphoid cells in the rabbit intestine, Gastroenterology 82:268–275.PubMedGoogle Scholar
  12. Hermans, P. E., Diaz-Buxo, J. A., and Stobo, J. D., 1976, Idiopathic late-onset immunog-lobulin deficiency: Clinical observations in 50 patients, Am. J. Med. 61:221–237.PubMedCrossRefGoogle Scholar
  13. Hughes, W. S., Cerda, J. J., Holtzapple, P., and Brooks, F. P., 1971, Primary hypogam-maglobulinemia and malabsorption, Ann. Intern. Med. 74:903–910.PubMedCrossRefGoogle Scholar
  14. Hurwitz, A. L., and Owen, R. L., 1978, Venereal transmission of intestinal parasites, West J. Med. 128:89–91.PubMedGoogle Scholar
  15. Lehrer, R. I., 1975, The fungicidal mechanisms of human monocytes. I. Evidence for myeloperoxidase-linked and myeloperoxidase-independent candidacidal mechanisms, J. Clin. Invest. 55:338–346.PubMedCrossRefGoogle Scholar
  16. Masur, H., Michelis, M. A., Green, J. B., Onorato, I., Vande Stouwe, R. A., Holzman, R. S. Warmser, G., Brettman, L., Lange, M., Murray, H. W., and Cunningham-Rundles, S., 1982, An outbreak of community-acquired Pneumocystis carinii pneumonia: Initial manifestation of cellular immune dysfunction, N. Engl.J. Med. 305:1431–1438.CrossRefGoogle Scholar
  17. Meyer, E. A., 1976, Giardia lamblia: Isolation and axenic cultivation, Exp. Parasitol. 39:101–105.PubMedCrossRefGoogle Scholar
  18. Meyers, J. D., Kuharic, H. A., and Holmes, K. K., 1977, Giardia lamblia infection in homosexual men, Br.J. Yener. Dis. 55:54–55.Google Scholar
  19. Moore, G. T., Cross, W. M., McGuire, D., Mollohan, C. S., Gleason, N. N., Healy, G. R., and Newton, L. H., 1969, Epidemic giardiasis at a ski resort, N. Engl. J. Med. 281:402–407.PubMedCrossRefGoogle Scholar
  20. Murray, H. W., and Cohn, Z. A., 1979 Macrophage oxygen-dependent antimicrobial activity. I. Susceptibility of Toxoplasma gondii to oxygen intermediates, J. Exp. Med. 150:938–949.PubMedCrossRefGoogle Scholar
  21. Olabuenaga, S. E., Cardoni, R. L., Segura, E. L., Fiera, N. E., and de Bracco, M. M. E., 1979, Antibody-dependent cytolysis of Trypanosoma cruzi by human polymorphonu-clear leukocytes, Cell. Immunol. 45:85–93.PubMedCrossRefGoogle Scholar
  22. Owen, R. L., Allen, C. L., and Stevens, D. P., 1979, Ultrastructural observations on giardiasis in a murine model. I. Intestinal distribution, attachment and relationship to the immune system of Giardia muris, Gastroenterology 76:757–759.PubMedGoogle Scholar
  23. Owen, R. L., Allen, C. L., and Stevens, D. P., 1981, Phagocytosis of murine Giardia muris by macrophages in Peyer’s patch epithelium, Infect. Immun. 33:591–601.PubMedGoogle Scholar
  24. Radulescu, S., and Meyer, E. A., 1981, Opsonization in vitro of Giardia lamblia trophozoites, Infect. Immun. 32:852–856.PubMedGoogle Scholar
  25. Roberts-Thomson, I. C, and Mitchell, G. F., 1978, Giardiasis in mice. I. Prolonged infections in certain mouse strains and hypothymic (nude) mice, Gastroenterology 75:42–46.PubMedGoogle Scholar
  26. Roberts-Thomson, I. C, Stevens, D. P., Mahmoud, A. A. F., and Warren, K. S., 1976, Acquired resistance to infection in an animal model of giardiasis, J. Immunol. 117:2036–2037.PubMedGoogle Scholar
  27. Saha, T. K., and Ghosh, T. K., 1977, Invasion of small intestinal mucosa by Giardia lamblia in man, Gastroenterology 72:402–405.PubMedGoogle Scholar
  28. Schmerin, M. J., Jones, T. C, and Klein, H., 1978, Giardiasis: Association with homosexuality, Ann. Intern. Med. 88:801–804.PubMedCrossRefGoogle Scholar
  29. Siegal, F. P., Lopez, C., Hammer, G. S., Brown, A. E., Kornfield, S. J., Gold, J., Hassett, J., Hirschman, S. F., Cunningham-Rundles, C., Adelsberg, B. R., Parham, D. M., Siegal, M., Cunningham-Rundles, S., and Armstrong, D., 1982, Severe acquired immunodeficiency in male homosexuals, manifested by chronic perianal ulcerative herpes simplex lesions, N. Engl. J. Med. 305:1439–1444.CrossRefGoogle Scholar
  30. Smith, P. D., Gillin, F. D., Brown, W. R., and Nash, T. E., 1981, IgG antibody to Giardia lamblia detected by enzyme-linked immunosorbent assay, Gastroenterology 80:1476–1480.PubMedGoogle Scholar
  31. Smith, P. D., Elson, C. O., Keister, D. B., and Nash, T. E., 1982a, Human host response to Giardia lamblia. I. Spontaneous killing by mononuclear leukocytes in vitro, J. Immunol. 128:1372–1376.PubMedGoogle Scholar
  32. Smith, P. D., Gillin, F. D., Kaushal, N. A., and Nash, T. E., 1982b, Antigenic analysis of Giardia lamblia from Afghanistan, Puerto Rico and Oregon, Infect. Immun. 36:714–719.PubMedGoogle Scholar
  33. Smith, P. D., Gillin, F. D., Spira, W. M., and Nash, T. E., 1982c, Chronic giardiasis: Studies on drug sensitivity, toxin production and host immune response, Gastroenterology 83:797–803.PubMedGoogle Scholar
  34. Smith, P. D., Keister, D. B., and Elson, C. O., 1983a, Human host response to Giardia lamblia. II. Antibody-dependent killing in vitro, Cell. Immunol. 82:308–315.PubMedCrossRefGoogle Scholar
  35. Smith, P. D., Elson, C. O., and Wahl, S., 1983b, Human monocyte recruitment by and phagocytosis of Giardia lamblia, Fed. Proc. 42:857.Google Scholar
  36. Stevens, D. P., Frank, D. P., and Mahmoud, A. A. F., 1978, Thymus dependency of host resistance to Giardia muris infection: Studies in nude mice, J. Immunol. 120:680–682.PubMedGoogle Scholar
  37. Visvesvara, G. S., Smith, P. D., Healy, G. R., and Brown, W. R., 1980, An immunofluo-rescence test to detect serum antibodies to Giardia lamblia, Ann. Intern. Med. 93:802–805.PubMedCrossRefGoogle Scholar
  38. Wright, S. G., and Tomkins, A. M., 1977, Quantification of the lymphocyte infiltrate in jejunal epithelium in giardiasis, Clin. Exp. Immunol. 29:408–412.PubMedGoogle Scholar
  39. Yardley, J. H., Takano, J., and Hendrix, T. R., 1964, Epithelial and other mucosal lesions of the jejunum in giardiasis: Jejunal biopsy studies, Bull. Johns Hopkins Hosp. 115:389–406.Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Phillip D. Smith
    • 1
  1. 1.Laboratory of Microbial ImmunityNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

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