New Conjugate Polymers — A Marriage Between Synthetic and Natural Polymers
Chemical modifications of enzymes such as Lipase or Tripsine to prepare novel enzyme conjugates were carried out by using telechelic oligo(N-isopropylacrylamide) which showed reversible dissolution-precipitation behaviors in water. The temperature-responsive enzyme conjugates retained the same enzymatic activities as native enzymes and recovery of the enzyme conjugates was possible by a simple temperature change to precipitate the enzyme conjugate out of the water solution and they were able to reuse in cycles without denaturation. This novel modulated systems is very useful for applications as a novel bioreactor system in comparison with immobilized enzyme systems.
KeywordsAcrylic Acid Native Enzyme Recycling System Enzyme Conjugate Butyl Methacrylate
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- 1.M. Heskins, J.E. Guillet, E. James, J. Macromol. Sci., Chem., A2, 1441–1455(1968).Google Scholar
- 4.T. Okano, N. Yui, M. Yokoyama, and R. Yoshida, Japanese Technol. Reviews Section E: Biotechnology, Vol. 4, No. 1, Gordon and Breach Science Publishers SA, Yverdon, Switzland.Google Scholar
- 12.M. Matsukata, Y. Takai, T. Aoki, K. Sanui, N. Ogata, Y. Sakurai, and T. Okano, J. Biochem., 116, 682–686(1994).Google Scholar