Phosphoprotein B-50 and Polyphosphoinositide-Dependent Signal Transduction in Brain
During the past several years, an impressive body of information has been accumulating that polyphosphoinositides (PPtdIns) metabolism plays a central role in cellular signal transduction (Berridge and Irvine, 1984). The prevailing view is that in response to receptor activation by a variety of hormones, neurotransmitters and other external stimuli, a plasma membrane pool of phosphatidylinositol-bisphosphate (PtdIns(4, 5)P 2) undergoes rapid phosphodiesteratic cleavage to yield two biologically active products: 1, 2 diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (InsP 3). Diacylglycerol is considered to activate the widely distributed Ca2+ and phospholipid-dependent protein kinase C which is capable of phosphorylating many, largely uncharacterized, cellular proteins, whereas InsP 3 is believed to interact to trigger the release of sequestered Ca2+ from non-mitochondrial sites into the cytosol. The simultaneous processes of protein phosphorylation and Ca2+ mobilization are thought to constitute synergistic events which are integral to a large number of cellular responses (Nishizuka, 1984).
KeywordsProtein Phosphorylation Inositol Phosphate Tetanic Stimulation Synaptic Plasma Membrane Synaptosomal Plasma Membrane
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