Phosphoprotein B-50 and Polyphosphoinositide-Dependent Signal Transduction in Brain

  • W. H. Gispen
  • P. N. E. de Graan
  • L. H. Schrama
  • J. Eichberg
Part of the FIDIA Research Series book series (FIDIA, volume 4)


During the past several years, an impressive body of information has been accumulating that polyphosphoinositides (PPtdIns) metabolism plays a central role in cellular signal transduction (Berridge and Irvine, 1984). The prevailing view is that in response to receptor activation by a variety of hormones, neurotransmitters and other external stimuli, a plasma membrane pool of phosphatidylinositol-bisphosphate (PtdIns(4, 5)P 2) undergoes rapid phosphodiesteratic cleavage to yield two biologically active products: 1, 2 diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (InsP 3). Diacylglycerol is considered to activate the widely distributed Ca2+ and phospholipid-dependent protein kinase C which is capable of phosphorylating many, largely uncharacterized, cellular proteins, whereas InsP 3 is believed to interact to trigger the release of sequestered Ca2+ from non-mitochondrial sites into the cytosol. The simultaneous processes of protein phosphorylation and Ca2+ mobilization are thought to constitute synergistic events which are integral to a large number of cellular responses (Nishizuka, 1984).


Protein Phosphorylation Inositol Phosphate Tetanic Stimulation Synaptic Plasma Membrane Synaptosomal Plasma Membrane 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • W. H. Gispen
    • 1
  • P. N. E. de Graan
    • 1
  • L. H. Schrama
    • 1
  • J. Eichberg
    • 1
    • 2
  1. 1.Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, Institute of Molecular BiologyState University of UtrechtUtrechtThe Netherlands
  2. 2.Department of Biochemical and Biophysical SciencesUniversity of HoustonHoustonUSA

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