Roles of Lipases in the Development of Autonomic Neurons

  • Fusao Hirata
  • Toshio Hattori
  • Yoshitada Notsu
  • Bernd Hamprecht
Conference paper
Part of the FIDIA Research Series book series (FIDIA, volume 4)


Glucocorticoids are widely used as therapeutics for immunological and inflammatory diseases (Sayers and Travis, 1970). This anti-inflammatory action of glucocorticoids is now proposed to be associated with the inhibition of the release of arachidonic acid, a precursor of inflammatory agents such as prostaglandins and leukotrienes (Blackwell et al., 1978). Arachidonic acid is mostly present in an esterified form rather than as a free fatty acid. Thus, the rate limiting step of the arachidonate release from intact cells is the activation of phospholipase A2 or phospholipase C. Furthermore, this anti-inflammatory action of glucocorticoids can be blocked by the previous treatment with cycloheximide and actinomycin D, inhibitors of protein and RNA syntheses, respectively (Hirata et al., 1985b). From these observations, one can assume that glucocorticoids induce the synthesis of a protein(s) which inhibits cellular phospholipases. Recently, the four groups (Flower’s group in England, Russo-Marie’s group in France, and Goldberg’s group and our group in USA) have partially purified and characterized such a factor(s) and named this protein as lipocortin (DiRosa et al., 1984). Isolated lipocortin can suppress the carageenin-induced paw edema, an animal model of acute inflammation, and can inhibit the release of arachidonic acid from various intact cells. Thus, it has been proposed that the inhibition of phospholipases A2 by lipocortin might be the main mechanism of the anti-inflammatory action of glucocorticoids (Hirata et al., 1985b).


Arachidonic Acid HL60 Cell Superior Cervical Ganglion Fluocinolon Acetonide Tyrosine Hydroxylase Activity 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Fusao Hirata
    • 1
  • Toshio Hattori
    • 1
  • Yoshitada Notsu
    • 1
  • Bernd Hamprecht
    • 2
  1. 1.Laboratory of Cell BiologyNational Institute of Mental HealthBethesdaUSA
  2. 2.Physiologisch-Chemisches Institut der Universität87 WurzburgWest Germany

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