NMR Studies of the Role of Intracellular Sodium Ions in the Mechanism of Insulin Action on an Amphibian Oocyte
NMR spectroscopy provides a noninvasive technique for the study of 23Na ions in living cells and tissues, permitting a direct observation of the cations via their own resonance absorption [1–10]. Rana oocytes constitute a particularly favorable cellular system for NMR study. A Rana female contains up to 2–3 thousand large (1.8 mm diameter) oocytes arrested in first meiotic prophase. Interestingly, insulin can release the block at prophase arrest in the amphibian oocyte and reinitiate the meiotic divisions in vitro, although its physiological role in vivo remains unclear. Insulin action on the plasma membrane causes a rapid change in ion permeability and electrical properties and we have arried out measurements to determine its effect on intracellular Na+ concentration [6,7].
KeywordsMeiotic Division None None Cell Water Shift Reagent Free Induction Decay Signal
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