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Application of Chemical Modification and Spin Labeling Techniques to the Study of Energy Conversion by Bacteriorhodopsin

  • L. Packer
  • A. T. Quintanilha
  • R. J. Mehlhorn

Abstract

Light generates a pH gradient and an electrical potential across the purple membrane of Halobacterium halobium. We are investigating the time resolved changes in protonation of the side chains of specific amino acid residues and the correlation of these changes with photon absorption and the ensuing photo-reaction cycle. We seek to determine the precise molecular description of the photocycle and of the time dependent steps in the uptake, translocation, and release of protons by the retinal proton catalyst in this membrane, bacteriorhodopsin (BR).

Keywords

Schiff Base Purple Membrane Proton Release Proton Movement Photostationary State 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • L. Packer
    • 1
  • A. T. Quintanilha
    • 1
  • R. J. Mehlhorn
    • 1
  1. 1.Dept. of Physiology/Anatomy; Membrane Bioenergetics Group Lawrence Berkeley LaboratoryUniversity of CaliforniaBerkeleyUSA

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