Hydration Dependent Lipid Phase Transitions in a Biological Membrane

  • John H. Crowe
  • Lois M. Crowe

Abstract

Phospholipids spontaneously form ordered structures in water, usually bilayers with the hydrocarbon chains in a fluid state, depending on the temperature, length of hydrocarbon chains, and the nature of the headgroup41,2). When these same phospholipids are dehydrated they undergo phase transitions to the gel state (again depending on the temperature) or to other crystalline states such as the well-known hexagonal II (HII) phase, in which the lipids are reorganized into tubes with the polar head groups oriented into the aqueous phase in the center of the tube. These phase transitions are well documented in pure phospholipid systems, but they have been poorly studied in complex mixtures of phospholipids or in intact biological membranes, even though there is some suggestion that such mixtures of phospholipids may exhibit different phase behavior from that of pure phospholipids (3). We have undertaken such a study, using isolated sarcoplasmic reticulum (SR) as a model membrane, and we report some of our progress in this regard in the present paper.

Keywords

Sarcoplasmic Reticulum Phase Behavior Hydrocarbon Chain Polar Head Group Freeze Fracture 
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References

  1. 1.
    D. Chapman, R.M. Williams, and B.D. Ladbrooke. Physical studies of phospholipids. VI. Thermotropic and lyotropic mesomorphism of some 1,2 diacyl-phosphatidylcholines (lecithins). Chem. Phys. Lipids 1: 445 (1967)CrossRefGoogle Scholar
  2. 2.
    J. Stumpel, E. Hansjorg, and A. Nicksch. X-ray analysis and calorimetry on phosphatidylcholine model membranes. The influence of length and position of acyl chains upon structure and phase behavior. Biochim. Biophys. Acta 727: 246 (1983).CrossRefGoogle Scholar
  3. 3.
    P.R. Cullis, G.W.M. Van Dijck, B. DeKruijff, and J. De Gier. Effect of cholesterol on the properties of equimolar mixtures of synthetic phosphatidylethanolamine and phosphatidylcholine. Biochim. Biophys. Acta 513: 21 (1978).PubMedCrossRefGoogle Scholar
  4. 4.
    H. Hauser. Water/phospholipid interactions. In: “Water Relations of Foods”, R.B. Duckworth, ed., Academic Press, London (1975).Google Scholar
  5. 5.
    M. Ruegg, U. Moor, A. Lukesch, and B. Blanc. Hydration and thermal stability of a-lactalbumín. A calorimetric study. In: “Application of Calorimetry in Life Science”, A. Lamprecht and B. Schaarschcoidt, eds., De Gruyter, Berlin (1977).Google Scholar
  6. 6.
    J.H. Crowe, L.M. Crowe, and S.J. O’Dell. Ice formation during freezing of Artemia cysts of variable water contents. Mol. Physiol. 1: 145 (1982).Google Scholar
  7. 7.
    C.-H. Chen, D.S. Berns, and A.S. Berns. Thermodynamics of carbohydrate-lipid interactions. Biophys. J. 36: 359 (1981).PubMedCrossRefGoogle Scholar
  8. 8.
    J. Seelig. 31P nuclear magnetic resonance and the head group structure of phospholipids in membranes. Biochim. Biophys. Acta 515: 105 (1978).PubMedCrossRefGoogle Scholar
  9. 9.
    J.H. Noggle, J.F. Marecek, S.B. Mandal, R. Van Venetie, J. Rogers, M.K. Jain, and F. Ramirez. Bilayers of phosphatidylglycerol and phosphatidylcholesterol give 31P NMR spectra characteristic for hexagonal and isotropic phases. Biochim. Biophys. Acta 691: 240 (1982).CrossRefGoogle Scholar
  10. 10.
    J.H. Crowe, and L.M. Crowe. Induction of anhydrobiosis: membrane changes during drying. Cryobiology 19: 317 (1982).PubMedCrossRefGoogle Scholar
  11. 11.
    J.H. Crowe and J.S. Clegg, eds. “Dry Biological Systems”, Academic Press, New York (1978).Google Scholar
  12. 12.
    J.H. Crowe, L.M. Crowe, and S.A. Jackson. Preservation of structural and functional activity in lyophilized sarcoplasmic reticulum. Arch. Biochem. Biophys. 220 (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • John H. Crowe
    • 1
  • Lois M. Crowe
    • 1
  1. 1.Department of ZoologyUniversity of CaliforniaDavisUSA

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