Volume Regulation in Carp Kidney Tissue as Influenced by Various Osmotic Agents

  • Ivan Beneš
  • Karel Janáček
  • Růžena Tauchová

Abstract

To what extent does the sodium pump (the plasma membrane Na,K-ATPase)contribute to volume regulation of animal cells? According to the original concept of Wilson (1954) and Leaf (1956) the sodium pump solves entirely the problem of volume maintenance in isotonic media, since “effectively impermeant” sodium ions (continually extruded by the pump) compensate for the Donnan excess of osmotic pressure, resulting mostly from the high concentration of diffusible counterions of macromolecules in the cytoplasm.

Keywords

Volume Regulation Tissue Slice Sodium Pump Osmotic Agent Frog Urinary Bladder 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Benes, I., Tauchova, R., and Janacek, K., 1982, Ouabain-insensitive mechanism of cell volume maintenance in the carp kidney, Physiol. boh.emoslov. 31: 237.Google Scholar
  2. Benes, I., Janacek, K., and Tauchovâ, R., 1983, Cell water content in carp kidney tissue slices as influenced by various osmotic agents, Physiol. bohemoslov., in press.Google Scholar
  3. Janacek, K., and Rybova, R., 1970, Nonpolarized frog bladder preparation - the effects of oxytocin, Pflügers Arch., 318: 294.PubMedCrossRefGoogle Scholar
  4. Janacek., K., Rybova, R., and Slavíkova, M., 1971, Independent stimulation of sodium entry and sodium extrusion in frog urinary bladder by aldosterone, Pflügers Arch., 326: 316.Google Scholar
  5. Kleinzeller, A., 1972, Cellular transport of water, in: “Metabolic Pathways: Metabolic Transport,” L. E. Hokin, ed.,Vol. 6, Academic Press, New York (1972).Google Scholar
  6. Leaf, A., 1956, On the mechanism of fluid exchange of tissues in vitro, Biochem. J., 62: 241.PubMedGoogle Scholar
  7. Ling, G. N., 1982, The physical state of K+ and water in the living cell according to the association-induction hypothesis and experimental evidence, in: Second International Conference on Water and Ions in Biological Systems, Abstracts, Bucharest.Google Scholar
  8. Macknight, A. D. C., and Leaf, A., 1974, Regulation of cellular volume, Physiol. Rev., 57: 510.Google Scholar
  9. Negendank, W., and Shaller, C., 1982, Isoosmotic volume regulation by human lymphocytes, in: Second International Conference on Water and Ions in Biological Systems, Abstracts, Bucharest.Google Scholar
  10. Wilson, T.H., 1954, Ionic permeability and osmotic swelling of cells, Science, 120: 104.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Ivan Beneš
    • 1
  • Karel Janáček
    • 1
  • Růžena Tauchová
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czechoslovakia

Personalised recommendations