The Intracavitary Basis of Solute Partitioning in Dextran (SephadexR) Gels and the Role of Vicinal Water

  • N. V. B. Marsden
  • A. Ch. Haglund


The partitioning of hydrocarbons and some of their hydroxyl derivatives is described. Two types of solute can be distinguished, non-polar or weakly polar, and polar. The former have an affinity for the gel which appears to involve a hydrophobic interaction and the affinity increases with increasing number of methylene carbons. Polar solutes on the other hand exhibit a reversed type of behaviour, i.e. steric exclusion. The partitioning of all solutes in all the gels can, however, be generalized into a single equation yielding for each gel a generalized logarithmic distribution coefficient. This suggests that although there are different partitioning mechanisms for different solutes, a common structural property of the gel may underlie all partitioning. The structural property best correlated with the generalized distribution coefficient is the concentration of ether oxygens a property which reflects how the matrix changes with increased cross-linking. On a basis of a comparison with the Schardinger cycloamyloses which have similar partitioning properties it is suggested that a cavitary structure is also required and that further, matrix perturbed (vicinal) water may be responsible for the hydrophobic interaction.


Polar Solute Polyhydric Alcohol Matrix Concentration Steric Exclusion Hydroxyl Derivative 
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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • N. V. B. Marsden
    • 1
  • A. Ch. Haglund
    • 1
  1. 1.Institute of Physiology and Medical BiophysicsUniversity of Uppsala, Biomedical CenterUppsalaSweden

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