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The Role of Water of Hydration in Acylation of Butyrylcholinesterase

  • Miloš R. Pavlič
  • Matjaž Zorko

Abstract

It was found recently in our laboratory (in preparation) that the acylation of butyrylcholinesterase (acylcholine acyl-hydrolase, EC 3.1.1.8) by methanesulfonylfluoride — a reaction analogous to the acylation of butyrylcholinesterase by its natural substrate — — is accelerated by tetramethylammonium and tetraethylammonium and that the accelerators improve the binding between the enzyme and the acylating agent. The same mechanism was found in our laboratory to be operative in the acceleration of acylation of acetylcholin-esterase (acetylcholine hydrolase, EC 3.1.1.7)1,2; in this case, the acceleration was shown to result from hydration changes in the active site of acetylcholinesterase under the influence of accelerator3–5.

Keywords

Natural Substrate Viscosity Coefficient Monovalent Cation Order Rate Constant Fast Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pavlič, M.R. and Wilson, I.B. (1978) Biochim. Biophys. Acta 523, 101–108.PubMedCrossRefGoogle Scholar
  2. 2.
    Pavlič, M.R. and Zorko, M. (1978) Biochim. Biophys. Acta 524, 340–348.PubMedCrossRefGoogle Scholar
  3. 3.
    Pavlič, M.R., in “Enzyme Regulation and Mechamism of Action” (P. Mildner and B. Ries, eds.) FEBS Vol. 60, Pergamon Press, Oxford and New York, 1980, pp. 113–122.Google Scholar
  4. 4.
    Pavlič, M.R. in “Synaptic Constituents in Health and Disease” ( M. Brzin, D. Sket and H. Bachelard, eds.) Mladinska knjiga and Pergamon Press, Ljubljana and Oxford, 1980, pp. 551–557.Google Scholar
  5. 5.
    Pavlič, M.R. (1980) Period. biol. 82, 449–453.Google Scholar
  6. 6.
    Ellman, G.L., Courtney, K.D., Andres, V.Jr. and Reatherstone, R.M. (1961) Biochem. Pharmacol. 7, 88–95.PubMedCrossRefGoogle Scholar
  7. 7.
    Monk, C.B. “Electrolytic Dissociation” Acad. Press, London and New York, 1961, p. 271.Google Scholar
  8. 8.
    Gordon, J.E. “The Organic Chemistry of Electrolyte solutions” John Willey and Sons, New York, London, Sidney and Toronto, 1975, p. 186, p. 197.Google Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Miloš R. Pavlič
    • 1
  • Matjaž Zorko
    • 1
  1. 1.Institute of BiochemistryMedical FacultyLjubljanaYugoslavia

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