Hydrate-H20 and Liquid Like H2O

H2O Interactions which are Necessary for Biological Activity
  • Hubertus Kleeberg
  • Werner A. P. Luck


H-bond interactions of H2O can be investigated with high accuracy in the region of the H2O combination vibration (near infrared (NIR): 1900–2000 nm or 5300–5000 cm−1).

Below 50 % relative humidity H2O forms H-bonds with the polar groups of biopolymers (Hydrate-H2O). This H2O is necessary for the stability of biomolecules but not sufficient for them to develop activity.

Above 50 % relative humidity liquid like H2O is present in addition to Hydrate-H2O.

The IR-spectrosopically determined amount of H2O which is changed in its intermolecular interactions in comparison to pure water is in agreement with the results of other methods. The consequences of biopolymer-H2O interactions are discussed with respect to enzymatic activity and partition coefficients in biological systems.


Pure Water Lone Electron Pair Collagenous System Triple Helical Structure Molecular Weight Solution 
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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Hubertus Kleeberg
    • 1
  • Werner A. P. Luck
    • 1
  1. 1.Department of Physical ChemistryUniversity of MarburgGermany

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