A Proposal to Study the Differentiation and Physiology of a Neuroblastoma: A Successful Research Grant Application Submitted to the National Institutes of Health
The differentiation and physiology of nervous tissue will be studied in vitro using cloned tissue culture lines of a mouse neuroblastoma as a model system. The parameters of the differentiation process will be defined with emphasis on control mechanisms and the induction of specific cytoplasmic and membrane proteins. Mutants which are blocked at the various steps in the differentiation process will be isolated. Similarly, the molecular biology of the cholinergic receptor and catecholamine uptake, storage, and release will be defined by studying the wild-type functions in relation to a series of mutants blocked in these functions. Finally, the nature of synaptic specificity will be studied with respect to membrane structure in electrical and, perhaps, chemical synapses in the neuroblastoma and other cloned tissue culture lines.
KeywordsTight Junction Acetylcholine Receptor Synaptic Vesicle Transmitter Release Mouse Neuroblastoma
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