The Stabilizing Protein, Protein-A, of Cysteine Dioxygenase
Cysteine dioxygenase [EC.18.104.22.168] catalyzes the oxygenation of cysteine to cysteine sulfinic acid, a key intermediate of cysteine metabolism to hypotaurine, taurine, isethionic acid and sulfate in mammalian tissues (1,3,8,10,11,18,19,22). The rat liver cysteine dioxygenase is obtained as an inactive form, which is activated by preincubation with L-cysteine under anaerobic condition, but the activated enzyme is rapidly and irreversibly inactivated during aerobic assay conditions. This inactivation during assay is prevented by a distinct cytoplasmic protein, called protein-A (13,14,18).
KeywordsHigh Protein Diet Standard Assay Condition Sulfur Amino Acid Hepatic Enzyme Activity Cysteine Sulfinic Acid
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